A
B
C
D
E
F
G
H
I
J
K
L
M

N
O
P
Q
R
S
T
U
V
W
X
Y
Z

Abbreviations:
SERUMplain clotted sample (serum) tube
EDTApotassium EDTA tube
LHlithium heparin tube
FLfluoride EDTA tube
CITtrisodium citrate tube
N/Anot applicable/available
Acetylcholine Receptor Antibody (Canine)
Suitable Sample:
PC
Unsuitable Sample:
LH
EDTA
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
15 working days
Request/Test Included in:
Request test
Comments:
Run at the Comparative Neuromuscular Laboratory, San Diego
Date Updated:
13/01/2017
Acid Fast Bacteria - culture
Suitable Sample:
Swab, fluid and/or fresh tissue PLUS Air-dried smear
Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
Ten (10) working days
Request/Test Included in:
Request test
Comments:
Does not include Johne's disease culture
Date Updated:
13/01/2017
Acid Fast Bacteria - microscopy
Suitable Sample:
Air-dried smear
Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
Daily
Request/Test Included in:
Comments:
Date Updated:
1/08/2007
ACTH (Endogenous ACTH)
Suitable Sample:
EDTA plasma (EDTA blood spun immediately after collection, plasma removed and placed in equal volumes into two plastic serum tubes and frozen immediately)
Unsuitable Sample:
PC
LH
Minimum Sample Size:
Turnaround Time:
15 working days
Request/Test Included in:
Request test
Comments:
PROTOCOL:
Preferably fast the animal overnight prior to collection.
Collect 5mL of blood into a plastic EDTA tube.
Immediately centrifuge the sample and remove the plasma and place equal volumes in two labelled plastic serum tubes and freeze immediately.
Transport one of these samples frozen (REQUEST PICK UP ON DRY ICE) to the laboratory and keep one sample frozen in case first sample thaws out in transport.
Date Updated:
5/9/2008
ACTH Stimulation Test (cortisol x 2)
Suitable Sample:
PC
Unsuitable Sample:
EDTA FL LH
Minimum Sample Size:
1.0 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
ACTH stimulation test (2 cortisol samples - Resting and 1 hour post injection)
Comments:
A single baseline cortisol is of minimal diagnostic value and dynamic tests of adrenal function are recommended for the diagnosis of both hypoadrenocorticism (Addisons) and hyperadrenocorticism (Cushings).
Date Updated:
13/01/2017
Adenovirus (Canine) antibody titre
Suitable Sample:
PC
Unsuitable Sample:
EDTA LH CIT FL
Minimum Sample Size:
3mL whole blood
Turnaround Time:
Approximately 2 weeks
Request/Test Included in:
Request Test
Comments:
Samples are sent on Monday and results are returned within two weeks. Can be done as an individual test, or as a combination (distemper, parvovirus, adenovirus)
Date Updated:
1/08/2007
Alanine aminotranferase (ALT)
Suitable Sample:
PC (preferred) LH EDTA FL
Unsuitable Sample:
Minimum Sample Size:
1mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Albumin
Suitable Sample:
Serum (preferred) LH EDTA FL
Unsuitable Sample:
Minimum Sample Size:
1mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Aldosterone
Suitable Sample:
Serum (Frozen)
Unsuitable Sample:
EDTA LH CIT FL
Minimum Sample Size:
1.0 mL of frozen serum
Turnaround Time:
Five (5) working days
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Alk Phos
Suitable Sample:
Serum LH
Unsuitable Sample:
EDTA CIT FL
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Alkaline phosphatase (ALP, Alk Phos)
Suitable Sample:
Serum LH
Unsuitable Sample:
EDTA CIT FL
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Allergy Test
Suitable Sample:
Serum
Unsuitable Sample:
EDTA LH
Minimum Sample Size:
3.0 mL whole blood
Turnaround Time:
Run once a week. Contact laboratory for expected time of completion of test.
Request/Test Included in:
Request test
Comments:
Measures IgE to a wide range of potential allergens (specific allergens).
Haemolysis interferes.
Antiinflammatory doses of corticosteroids should not significantly suppress IgE production. However, immunosuppressive doses (or other immunosuppressants) will reduce Ab levels.
Date Updated:
1/08/2007
ALP
Suitable Sample:
Serum LH
Unsuitable Sample:
EDTA CIT FL
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
ALT: Alanine aminotransferase
Suitable Sample:
Serum (preferred) LH EDTA FL
Unsuitable Sample:
Minimum Sample Size:
1mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Amikacin
Suitable Sample:
Serum
Unsuitable Sample:
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Two (2) working days
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Amylase
Suitable Sample:
SERUM LH
Unsuitable Sample:
EDTA FL
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
1 hour
Request/Test Included in:
Request test
CP1, 2, 5, 6
Comments:
Date Updated:
1/08/2007
ANA (Anti-Nuclear Antibody) (Equine)
Suitable Sample:
PC
Unsuitable Sample:
LH EDTA
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
2 working days (test not available from Christmas until the New Year)
Request/Test Included in:
Request test
Comments:
Positive at a titre of >1:10 in 90% of SLE cases, but is not highly specific and false positive will occur (but usually at low titres)
Date Updated:
1/08/2007
ANA (Antinuclear Antibody) by IFA (Canine)
Suitable Sample:
PC (canine only)
Unsuitable Sample:
EDTA LH
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Run Tuesday & Friday Cut off time 9:00 am
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Anti-acetylcholine receptor antibody
Suitable Sample:
PC
Unsuitable Sample:
EDTA LH CIT
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Ten (10) working days (allow at least 2 weeks)
Request/Test Included in:
Request test
Comments:
Diagnostic test for myasthenia gravis
Date Updated:
1/08/2007
Anticoagulant rodenticides+
Suitable Sample:
For postmortem samples liver is the best sample (preferably frozen) Vomitus - preferably frozen
Unsuitable Sample:
Blood, urine
Minimum Sample Size:
10 gm minimum, but the more the better
Turnaround Time:
Allow 6 weeks (contact laboratory to confirm expected time for availability of results)
Request/Test Included in:
Request test
Comments:
Quantitative assays for brodifacoum, bromodiolone, warfarin, coumatetrallyl and pindone
Date Updated:
1/08/2007
Arsenic - acute (exposure in last few days)
Suitable Sample:
PC or Urine
Unsuitable Sample:
Minimum Sample Size:
10 mL whole blood 10 mL urine
Turnaround Time:
Ten (10) working days (2 weeks)
Request/Test Included in:
Request test
Comments:
Acute exposure = in the last few days
Date Updated:
1/08/2007
Arsenic - chronic (6-12 months)
Suitable Sample:
Hair or claws
Unsuitable Sample:
Blood
Minimum Sample Size:
1 x hair 2 gm claw
Turnaround Time:
Ten (10) working days
Request/Test Included in:
Request test
Comments:
Chronic exposure = 6 - 12 months
Date Updated:
1/08/2007
Aspartate aminotransferase (AST)
Suitable Sample:
PC LH EDTA (not recommended)
Unsuitable Sample:
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
2 hours
Request/Test Included in:
Request test CP 2, 6 FP 2, 5 Canine, feline & ruminant liver monitoring panels Phenobarbitone monitoring panel
Comments:
AST may be from either skeletal muscle or liver origin
To assess muscle damage, measure both CK and AST
AST is also present in RBC's and activity may be higher in haemolysed samples
Date Updated:
1/08/2007
Aspergillus Serology
Suitable Sample:
PC (non-haemolysed)
Unsuitable Sample:
EDTA LH
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
3 working days
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Aspirin
Suitable Sample:
PC
Unsuitable Sample:
EDTA
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Two (2) working days
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
AST: Aspartate amino transferase+
Suitable Sample:
PC LH EDTA (not recommended)
Unsuitable Sample:
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
2 hours
Request/Test Included in:
Request test CP 2, 6 FP 2, 5 Canine, feline & ruminant liver monitoring panels Phenobarbitone monitoring panel
Comments:
AST may be from either skeletal muscle or liver origin
To assess muscle damage, measure both CK and AST
AST is also present in RBC's and activity may be higher in haemolysed samples
Date Updated:
1/08/2007
Avian Biochemistry
Suitable Sample:
PC
Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
Daily
Request/Test Included in:
Preferred analytes in order of preference
Comments:
Date Updated:
1/08/2007
Avian Haematology
Suitable Sample:
Air-dried smear
Unsuitable Sample:
LH
Minimum Sample Size:
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/08/2007
Avian Profile
Suitable Sample:
Air-dried smear LH PC
Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
Daily
Request/Test Included in:
Comments:
Preferred biochemistry analytes in order of preference
Date Updated:
1/08/2007
Babesia gibsonii Export Testing (IFA and Smears)
Suitable Sample:
SERUM and capillary blood smears
Unsuitable Sample:
EDTA blood
Minimum Sample Size:
2 mL whole blood 2 blood smears made from ear margin capillary blood
Turnaround Time:
Tests are run on Monday (or next working day after public holiday) and Thursday at Vetpath
Cutoff time 9:00 am at Vetpath
Results are reported out later the same day.
Request/Test Included in:
Request test
Comments:
See Vetpath website for current information. http://www.vetpath.com.au If the sample is going to be in transit for more than a day, it is recommended that the serum is separated off if possible and the serum rather than whole is sent. (This minimises haemolysis.)
Date Updated:
1/10/2007
Beak and Feather Disease (PBFD, Circovirus) by PCR
Suitable Sample:
At least 3 freshly plucked feathers. These should be sealed in a small plastic bag or container. and/or LH blood: the volume should reflect the size of the bird, only a few drops are required for smaller species.
Unsuitable Sample:
EDTA SERUM
Minimum Sample Size:
Blood: volume of LH blood depends on size of bird, a few drops from smaller species is acceptable. Feathers: at least 3 freshly plucked feathers (send in small plastic bag or container). A few drops of LH blood is acceptable from small species of birds.
Turnaround Time:
Approximately 2 weeks (confirm with laboratory)
Request/Test Included in:
Request test
Comments:
PCR test
Date Updated:
1/10/2007
Betahydroxybutyrate
Suitable Sample:
SERUM
Unsuitable Sample:
Minimum Sample Size:
1mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test If the glucose is elevated in a profile, the BOHB is automatically included.
Comments:
Date Updated:
27/08/2008
Bicarbonate
Suitable Sample:
SERUM LH
Unsuitable Sample:
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Bile Acid
Suitable Sample:
SERUM EDTA
Unsuitable Sample:
LH (interferes)
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Protocol for dogs and cats: 1. Overnight fast 2. Collect a baseline blood sample (plain clotted) Some healthy Maltese and possibly Maltese cross dogs have elevated SBA without having hepatic dysfunction. Elevated SBA are difficult to interpret in these dogs.
Date Updated:
15/03/2016
Bile Acids (serum bile acids x 2)
Suitable Sample:
SERUM EDTA
Unsuitable Sample:
LH (interferes)
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Protocol for dogs and cats:
1. Overnight fast.
2. Collect a baseline blood sample (plain clotted)
3. Immediately feed a moderately fatty meal. If inappetant, a small amount of oil can be syringe-fed (the intent is to stimulate gall bladder contraction).
4. Collect a second blood sample two hours after the first sample.
Some healthy Maltese and possibly Maltese cross dogs have elevated SBA without having hepatic dysfunction. Elevated SBA are difficult to interpret in these dogs.
Date Updated:
1/10/2007
Bilirubin (total bilirubin)
Suitable Sample:
SERUM LH EDTA
Unsuitable Sample:
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
15/07/2008
Biochemistry - single tests
Suitable Sample:
SERUM LH
Unsuitable Sample:
EDTA (can do some tests, see comments)
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test by name
Comments:
If only EDTA is submitted:
Test that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
Test that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorous, HCO3

If more than two or three tests, look to see if there is a profile or monitoring panel that is more economic.
Date Updated:
1/10/2007
Biochemistry Profile (VBA)
Suitable Sample:
SERUM LH
Unsuitable Sample:
EDTA (can do some tests, see comments)
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
If only EDTA is submitted:

Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
Tests that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorous, HC)3
Date Updated:
1/10/2007
Biochemistry Profile (VBA/ Amylase/ Lipase)
Suitable Sample:
SERUM LH
Unsuitable Sample:
EDTA (can do some tests, see comments section)
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
If only EDTA is submitted:

Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
Tests that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorus, HCO3
Date Updated:
1/10/2007
Biopsy
Suitable Sample:
Tissue in 10x volume of buffered formalin
Unsuitable Sample:
Tissue in any other fluid
Minimum Sample Size:
Tissue in 10x volume of buffered formalin
Turnaround Time:
Two (2) - Three (3) Working days
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Bitch Pregnancy (Relaxin)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA CIT LH FL
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Sample should be collected at 30 days+
This test can be used in the cat.
Date Updated:
8/07/2008
Blood Culture
Suitable Sample:
1-5 mls of non-anticoagulated blood in yellow blood culture bottle (request from laboratory). Do not refrigerate the sample.
Unsuitable Sample:
Blood collected into any other container
Minimum Sample Size:
1 mL blood
Turnaround Time:
Cultures are examined at 36 hrs(interim report) and 5 days(final report).
Request/Test Included in:
Request test
Comments:
For optimal chances of a positive culture, collect 3 separate samples over a 24 hour period.
Do not refrigerate.
Contact Vetnostics for a blood culture bottle- supplied individually as expensive and rapidly expire.
Pediatric blood culture bottle required as can culture aerobically and anaerobically from them.
Date Updated:
30/07/2008
Blood Grouping (Feline)
Suitable Sample:
EDTA-preferred. CIT-possible
Unsuitable Sample:
SERUM LH
Minimum Sample Size:
Absolute minimum: 30-40 uL
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
15/07/2008
Blood Lead
Suitable Sample:
EDTA LH
Unsuitable Sample:
SERUM
Minimum Sample Size:
1mL whole blood
Turnaround Time:
Monday, Wednesday and Friday
Request/Test Included in:
Request test
Comments:
Date Updated:
15/07/2008
Blood Typing (Canine -DEA 1.1)
Suitable Sample:
EDTA-preferred. CIT-possible.
Unsuitable Sample:
SERUM LH
Minimum Sample Size:
0.5 mL
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
15/07/2008
Body fluid analysis & cytology
Suitable Sample:
EDTA AND smears from site
Unsuitable Sample:
Minimum Sample Size:
1-2 mL
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
BOHB (B-hydroxybutyrate)
Suitable Sample:
SERUM
Unsuitable Sample:
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test If the glucose is elevated in a profile, the BOHB is automatically included.
Comments:
Date Updated:
27/08/2008
Bone Marrow Cytology (includes CBC)
Suitable Sample:
EDTA-anticoagulated bone marrow OR Smears from bone marros PLUS EDTA-anticoagulated blood
Unsuitable Sample:
SERUM LH
Minimum Sample Size:
0.5 mL bone marrow (non-clotted) 1.0 mL EDTA peripheral blood
Turnaround Time:
Within 24 hours of arrival Monday to Thursday.
Request/Test Included in:
Request test
Comments:
It is important to have a concurrent peripheral blood to interpret the bone marrow cytology. The cost of a CBC is included in the fee for bone marrow cytology.
Clotting is frequently a problem with bone marrow samples and the syringe used for aspiration must contain sufficient EDTA to prevent this.
Date Updated:
1/10/2007
Brodifacoum (rodenticide)
Suitable Sample:
For postmortem samples liver is the best sample (preferably frozen) Vomitus - preferably frozen
Unsuitable Sample:
Blood Urine
Minimum Sample Size:
10 gm minimum, but the more the better
Turnaround Time:
Allow <6 weeks (contact laboratory to confirm expected time for availability of results).
Request/Test Included in:
Request test
Comments:
Quantitative assays for brodifacoum, bromodiolone, warfarin, coumatetrallyl and pindone
Date Updated:
1/10/2007
Bromide (Potassium Bromide)
Suitable Sample:
SERUM (without serum separator gel)
Unsuitable Sample:
EDTA CIT FL
Minimum Sample Size:
0.5 mL serum
Turnaround Time:
Two (2) - Three (3) Working days
Request/Test Included in:
Request test
Comments:
Collect sample 1 hour prior to the next dose of KBr
Mild lipaemia and/or haemolysis is acceptable.
Date Updated:
21/07/2008
Bromide and Phenobarbitone
Suitable Sample:
SERUM (without serum separator gel)
Unsuitable Sample:
EDTA CIT FL
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
FOR BROMIDE: Two (2) - Three (3) Working days FOR PHENOBARBITAL: Daily.
Request/Test Included in:
Request test
Comments:
FOR BROMIDE: Collect sample 1 hour prior to the next dose of KBr (Mild lipaemia and/or haemolysis is acceptable).
FOR PHENOBARBITAL: Collect sample at any time between doses (Thiopentone anaesthesia does not interfere).
Date Updated:
24/07/2008
Bronchoalveolar Lavage (BAL)
Suitable Sample:
Sterile urine pot (can also collect some into EDTA if for cytology only)
Unsuitable Sample:
Minimum Sample Size:
1mL (small animals)
Turnaround Time:
24 hours Monday-Saturday
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Brucella canis (for export)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH
Minimum Sample Size:
2 - 3 mL whole blood (need 1 mL serum)
Turnaround Time:
Eight (8) Working days
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Calcium (Ca)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA FL CIT
Minimum Sample Size:
2mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
May be affected by haemolysis and/or lipaemia
Date Updated:
14/08/2008
Calcium (Ca) - Ionised
Suitable Sample:
SERUM - using Serum Separator Tube (BD Vacutainer tube SSTII)
Unsuitable Sample:
LH CIT
Minimum Sample Size:
The minimum volume of serum required for ionised calcium testing is 0.75 mls of serum ( but a greater volume is preferred).
Turnaround Time:
Within 12 hours from arrival at laboratory
Request/Test Included in:
Request test
Comments:
SST (serum separation tubes which contain gel) vacutainer tubes should be used into which a whole blood sample (of at least 2.5-5 mls) should be placed (through stopper, without uncapping lid) and centrifuged 30 minutes after collection.
Make sure that SST sample tube is not uncapped at any stage prior to testing for ionised calcium at the lab. Keep sample refrigerated, no requirement to freeze.
Please submit separately to any other requests (separate submission form and separate sample bag).
Date Updated:
22/06/2017
Calculi Identification
Suitable Sample:
Calculi in sterile container.
Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Calicivirus - Rabbit
Suitable Sample:
Liver (fresh or frozen)
Unsuitable Sample:
Blood
Minimum Sample Size:
Amount not stated
Turnaround Time:
Run once weekly, allow 5-10 working days
Request/Test Included in:
Request test
Comments:
Test detects antigen
Date Updated:
1/10/2007
Calicivirus (Feline) by PCR
Suitable Sample:
Swab of tears or exudate (see Comments)
Unsuitable Sample:
Sample in transport medium. Frozen sample.
Minimum Sample Size:
Single swab.
Turnaround Time:
Eight (8) working days.
Request/Test Included in:
Feline Upper Respiratory Tract Infection by PCR
Comments:
Use a dry sterile cotton swab to collect the sample.
Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs or the appropriate lesion.
Place the swab in a sterile container and refrigerate.
Do not freeze.
Do not place in transport medium.
Date Updated:
18/08/2008
Canine Adenovirus Antibody Titre by VN (Infectious Canine Hepatitis)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH CIT FL
Minimum Sample Size:
3 mL whole blood
Turnaround Time:
Approximately 2 weeks
Request/Test Included in:
Request Test
Comments:
Samples are sent on Mondays and results are returned within two weeks.
Date Updated:
1/10/2007
Canine Allergy Screen
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH
Minimum Sample Size:
3.0 mL whole blood
Turnaround Time:
Run once a week
Request/Test Included in:
Request Test
Comments:
Greer Allergy Elisa
Measures IgE to a wide range of potential allergens (specific allergens).
Haemolysis interferes.
Antiinflammatory doses of corticosteroids should not significantly suppress IgE production. However, immunosuppressive doses (or other immunosuppressants) will reduce Ab levels.
Date Updated:
15/08/2008
Canine Blood Typing (DEA1.1)
Suitable Sample:
EDTA-preferred; citrate -possible.
Unsuitable Sample:
SERUM LH
Minimum Sample Size:
0.5 mL
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
15/08/2008
Canine Distemper Virus & Parvovirus Antibody Titres by IFA
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH CIT
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
8 working days
Request/Test Included in:
Combined CDV & CPV IFA titres The tests can also be requested individually
Comments:
Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations. Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.
Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests. Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV.
INTERPRETATION:
Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
Titres of >1:5 are considered seropositive.
Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
Dogs with titres of >1:20 probably do not require revaccination.
Date Updated:
18/07/2008
Canine Distemper Virus Antibody Titre by IFA
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH CIT
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Eight (8) working days.
Request/Test Included in:
CDV antibody titres by IFA
Comments:
Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations.Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.
Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests.Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV.
INTERPRETATION:
Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
Titres of >1:5 are considered seropositive.
Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
Dogs with titres of >1:20 probably do not require revaccination.
Date Updated:
1/10/2007
Canine Hepatitis Antibody
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH CIT FL
Minimum Sample Size:
3 mL whole blood
Turnaround Time:
Approximately 2 weeks
Request/Test Included in:
Request test
Comments:
Samples are sent on Mondays and results are returned within two weeks.
Date Updated:
1/10/2007
Canine Parvovirus Antigen (Faeces)
Suitable Sample:
Faeces Faecal swab
Unsuitable Sample:
Blood
Minimum Sample Size:
Faecal swab
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
False negative results may occur as the period of viral shedding in the faeces is relatively brief and virus is seldom detected 10 to 12 days after infection (this corresponds to 5 to 7 days of clinical illness).
False negatives may also occur very early in infection; retest in 36 to 48 hours if this is suspected.
Modified live vaccination results in transient faecal shedding and may give a weak positive result 5-15 days after vaccination.
Date Updated:
1/10/2007
Canine ParvovirusAntibody Titre by IFA
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
10 working days
Request/Test Included in:
CPV antibody titre by IFA. Combined CDV & CPA IFA titres can also be requested.
Comments:
Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations. Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease. Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests. Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV. INTERPRETATION: Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended. Titres of >1:5 are considered seropositive. Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased. Dogs with titres of >1:20 probably do not require revaccination.
Date Updated:
24/03/2016
Canine TLI
Suitable Sample:
SERUM (canine only) An overnight (>6 hours) fast is recommended prior to blood collection
Unsuitable Sample:
EDTA LH FL CIT Species other than canine
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Run Monday, Wednesday and Friday Results are available the same day
Request/Test Included in:
Request test
Comments:
Sensitive and specific test for the diagnosis of canine exocrine pancreatic insufficiency (EPI)
Reference range for healthy dogs is 5.4 - 32 ng/mL
Values <2.5 ng/mL are diagnostic for canine exocrine pancreatic insufficiency.
Dogs with values 2.5 - 5.0 ng/mL should be retested in one month.
Administration of oral pancreatic extracts does not affect serum TLI concentrations in normal dogs or dogs affected with EPI. Consequently withdrawal of enzyme supplementation is unnecessary.
Date Updated:
18/07/2008
Cardiac troponin I
Suitable Sample:
SERUM (gel tubes OK) LH (will give slightly lower results than serum) If monitoring, it is important to use the same sample type and same type of tubes for each sample
Unsuitable Sample:
EDTA
Minimum Sample Size:
1.0 mL whole blood
Turnaround Time:
Daily - Monday - Friday
Request/Test Included in:
Request test
Comments:
This is presently an unvalidated test in equine, canine and feline.
Troponin I in serum increases in response to myocardial injury.
Date Updated:
26/07/2008
CBC (Complete Blood Count)
Suitable Sample:
EDTA CIT if <1 day old - can be done as a last option LH for some birds and reptiles
Unsuitable Sample:
SERUM
Minimum Sample Size:
1.0 mL, but prefer 2 mL
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
LH can be used for a CBC, but platelets and WBCs clump and the fibrinogen cannot be done.
Date Updated:
18/07/2008
Ceroid Lipofuscinosis (PCR test, B Collies)
Suitable Sample:
EDTA
Unsuitable Sample:
Minimum Sample Size:
1ml whole blood
Turnaround Time:
Eight (8) working days.
Request/Test Included in:
Request test
Comments:
Specifically for Border Collies
Date Updated:
18/08/2008
Chlamydia - Avian by PCR
Suitable Sample:
Conjunctival swab AND/OR Cloacal swab See below for sample collection
Unsuitable Sample:
Sample in transport medium. Frozen sample.
Minimum Sample Size:
Single swab.
Turnaround Time:
Eight (8) working days.
Request/Test Included in:
Chlamydia PCR for Avian
Comments:
Use a dry sterile cotton swab to collect the sample.
Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs and/or cloaca.
Place the swab in a sterile container and refrigerate.
Use a separate container for each animal if collecting from more than one animal.
Do not freeze.
Do not place in transport medium.
Date Updated:
1/10/2007
Chlamydia - Feline by PCR
Suitable Sample:
Swab of tears or exudate (see Comments)
Unsuitable Sample:
Sample in transport medium. Frozen sample.
Minimum Sample Size:
Single swab.
Turnaround Time:
Eight (8) working days.
Request/Test Included in:
Chlamydia PCR for felines
Comments:
Use a dry sterile cotton swab to collect the sample.
Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs or the appropriate lesion.
Place the swab in a sterile container and refrigerate.
Do not freeze.
Do not place in transport medium.
THE PCR LAB does not recommend testing feline samples for Chlamydia only as it is difficult to distinguish between feline herpesvirus, feline calicivirus and chlamydia infections based on clinical symptoms.
Date Updated:
18/08/2008
Chlamydia - Koala by PCR
Suitable Sample:
Conjunctival swab AND/OR Cloacal swab See below for sample collection
Unsuitable Sample:
Blood Swabs in transport medium
Minimum Sample Size:
One swab If more than one swab is submitted per animal, each swab is charged for separately
Turnaround Time:
Eight (8) working days.
Request/Test Included in:
Chlamydia PCR for koalas
Comments:
Use a dry sterile cotton swab to collect the sample.
Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs and/or cloaca.
Place the swab in a sterile container and refrigerate.
Use a separate container for each animal if collecting from more than one animal.
Do not freeze.
Do not place in transport medium.
Date Updated:
1/10/2007
Chloride
Suitable Sample:
SERUM LH
Unsuitable Sample:
EDTA FL
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Cholesterol
Suitable Sample:
SERUM
Unsuitable Sample:
CIT FL oxalate
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
18/08/2008
Cholinesterase
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA CIT FL oxalate
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Reduced nonspecific esterase activity in the blood is an indicator of exposure to organophosphates.
Date Updated:
18/08/2008
CK (Creatine Kinase)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA FL
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
To assess muscle damage usually measure CK and AST.
Date Updated:
18/08/2008
Clostridium difficile Toxin A
Suitable Sample:
Faeces
Unsuitable Sample:
Minimum Sample Size:
1 gram
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
18/08/2008
Clotting Times (PT, APTT, TT) and fibrinogen
Suitable Sample:
CIT
Unsuitable Sample:
SERUM EDTA LH
Minimum Sample Size:
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Beware of insufficiently filled OR overfilled CIT tubes; it is important to fill exactly to the mark on the tubes. Beware of expired tubes.
Date Updated:
15/03/2016
Coagulation Screen (CBC, PT, APTT, TT fibrinogen, platelets, smear)
Suitable Sample:
EDTA AND CIT
Unsuitable Sample:
SERUM LH
Minimum Sample Size:
Filled CIT tube PLUS 2 mL EDTA
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Beware of insufficiently filled OR overfilled CIT tubes; tubes must be filled to just the line marked on the tube.
Beware of clots in the sample.
Date Updated:
18/08/2008
Complete blood count (CBC)
Suitable Sample:
EDTA CIT if <1 day old - can be done as a last option LH for some birds and reptiles
Unsuitable Sample:
SERUM
Minimum Sample Size:
1.0 mL, but prefer 2 mL
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
LH can be used for a CBC, but platelets and WBCs clump and the fibrinogen cannot be done.
Date Updated:
18/07/2008
Coomb's Test (Canine, Feline )
Suitable Sample:
EDTA
Unsuitable Sample:
SERUM
Minimum Sample Size:
0.5 mL whole blood
Turnaround Time:
Daily - Monday to Saturday
Request/Test Included in:
Request test
Comments:
NOTE: Coombs tests are species specific
Date Updated:
1/10/2007
Coomb's Test (Equine)
Suitable Sample:
Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
Request/Test Included in:
Comments:
Equine Coomb's test is no longer available
Date Updated:
10/01/2017
Copper (large animals)
Suitable Sample:
LH Tissue - liver if deficiency suspected Tissue - liver or kidney if excess is suspected
Unsuitable Sample:
SERUM EDTA
Minimum Sample Size:
10 mL whole blood 100 gm fresh tissue (can be frozen)
Turnaround Time:
Ten (10) working days.
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Copper (small animals, birds)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA
Minimum Sample Size:
1 mL whole blood
Turnaround Time:
Run Tuesday and Thursday
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Coronavirus - Immunocytochemistry
Suitable Sample:
effusion fluid
Unsuitable Sample:
SERUM EDTA CIT
Minimum Sample Size:
2 mL fluid 10 mL of fluid is preferable
Turnaround Time:
Two (2) working days
Request/Test Included in:
Request test
Comments:
This is a highly specific test to demonstrate FIP-related antigens in macrophages in effusion fluid.

The anti-FCoV direct IFA test detects direct immunofluorescent staining of IP-related antigens in macrophages in the effusion with anti-FCoV antibodies. This is a very specific diagnostic test as only FIP-infected cats will have virus-infected macrophages. This test has a specificity of 1.00 (100% positive predictive value) but a relatively low sensitivity of 0.57.
No single test is able to confirm a diagnosis of FIP apart from histopathology. Clinical diagnosis of FIP should be based on a wide variety of tests including history, age, number of cats in household, persistent pyrexia and laboratory data including assessment of acute phase proteins, analysis of effusions, haematology and biochemical tests as well as specific serological tests to list a few.
A fluid analysis is required of the effusion prior to this test being performed.
Date Updated:
1/10/2007
Coronavirus - Qualitative Antibody
Suitable Sample:
SERUM
Unsuitable Sample:
Minimum Sample Size:
0.5 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
This test detects antibodies against Coronaviruses (both pathogenic and nonpathogenic).
Date Updated:
18/08/2008
Coronavirus Antibody Titre by IFA (Feline)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA LH Species other than cats
Minimum Sample Size:
1.0 mL whole blood
Turnaround Time:
Four (4) working days.
Request/Test Included in:
Request test
Comments:
This is the gold-standard test for measurement of antibodies to feline coronavirus (FCoV).
Titres of 1:10 or less are considered seronegative
Titres 1:40 or greater considered seropositive
Titres <1:10 indicate a low likelihood of coronavirus shedding. If the cat is infected they are likely to stop shedding when isolated.
Cats with high titres (1:640 or higher) are almost always shedding virus but can stop shedding upon isolation. This can be demonstrated by a drop in their titre to low or undetectable levels.
Cats with clinical FIP usually have a high titre (1:640 or higher) although a few cases may show low titres in the terminal stages (particularly in the effusive forms).
The higher the titre the greater the specificity and sensitivity of this test for a diagnosis of FIP. The positive predictive index at 1:1600 is 0.94 and the negative predictive index 0.88 (J Vet Int Med 2003:17 781-790)
No single test is suitable to confirm a diagnosis of FIP apart from histopathology. Clinical diagnosis of FIP should be based on a wide variety of tests including history, age, number of cats in household, persistent pyrexia and laboratory data including assessment of acute phase proteins, analysis of effusions, haematology and biochemical tests as well as specific serological tests to list a few.
NOTE: This test can be done on feline or canine serum, but there are no guidelines for interpretation of canine results and it is not recommended for this species.
Date Updated:
1/10/2007
Cortisol
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA FL LH
Minimum Sample Size:
1.0 mL whole blood
Turnaround Time:
Daily - Monday
Request/Test Included in:
Cortisol ACTH stimulation test (2 cortisol samples) to Saturdays Low dose dexamethasone suppression test (3 cortisol samples)
Comments:
A single cortisol is of minimal diagnostic value and dynamic tests of adrenal function are recommended for the diagnosis of both hypoadrenocorticism (Addisons) and hyperadrenocorticism (Cushings) - ie. ACTH stimulation test or Low Dose Dexamethasone Suppression test (LDDST).
Date Updated:
1/10/2007
Cortisol - Equine
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA FL LH
Minimum Sample Size:
3 mL whole blood
Turnaround Time:
Eight (8) working days.
Request/Test Included in:
Request test
Comments:
Date Updated:
18/08/2008
Creatine Kinase (CK)
Suitable Sample:
SERUM
Unsuitable Sample:
EDTA FL
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
To assess muscle damage usually measure CK and AST.
Date Updated:
18/08/2008
Creatinine
Suitable Sample:
SERUM
Unsuitable Sample:
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Date Updated:
30/07/2008
Cross Match (Saline cross match only - not suitable for horses)
Suitable Sample:
SERUM from both donor and recipient EDTA from both donor and recipient
Unsuitable Sample:
LH
Minimum Sample Size:
2 mL whole blood in EDTA tubes from donor and recipient 2 mL whole blood in SERUM tubes from donor and recipient
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Avoid haemolysis as 2+ haemolysis gives invalid results.
Major cross-match = donor RBCs + recipient serum
Minor cross match = recipient RBCs + donor serum
Date Updated:
18/07/2008
Cryptococcus Antigen Titre
Suitable Sample:
SERUM CSF
Unsuitable Sample:
EDTA LH
Minimum Sample Size:
2 mL whole blood
Turnaround Time:
Two (2) Working days
Request/Test Included in:
Request test
Comments:
Titration is essential in positive cases to monitor the response to treatment
Positive predictive value in serum is >85%
Positive predictive value in CSF is >98%.
Date Updated:
1/10/2007
Cryptosporidium (Dogs and Cats)
Suitable Sample:
Fresh faeces The sample can be stored for up to 2 days at 2-8°C
Unsuitable Sample:
Blood
Minimum Sample Size:
1-2 gram (small volume)
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Detects Cryptosporidium parvum in animal faeces with reported sensitivity is 96.7% and specificity is 99.9%
Detects Giardia duodenalsis antigen in dog and cat faeces with a reported sensitivity 97.2% and specificity 99.9%
Date Updated:
6/08/2008
CSF (Cerebrospinal Fluid)
Suitable Sample:
Sample in EDTA or SERUM tube (1-2 ml maximum needed from dogs and cats) EDTA is suitable for cytology, but is not suitable for culture. If using plain tubes, do NOT use tubes with gel activator in them. Discuss with laboratory
Unsuitable Sample:
LH
Minimum Sample Size:
>0.25mL (preferably >0.5 mL)
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Should reach the laboratory within 2-4 hours, but if not possible keep cool in transit.
Date Updated:
30/07/2008
CSF analysis and cytology
Suitable Sample:
Sample in EDTA or SERUM tube (1-2 ml maximum needed from dogs and cats) EDTA is suitable for cytology, but is not suitable for culture. If using plain tubes, do NOT use tubes with gel activator in them. Discuss with laboratory
Unsuitable Sample:
LH
Minimum Sample Size:
>0.25mL (preferably >0.5 mL)
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Should reach the laboratory within 2-4 hours, but if not possible keep cool in transit.
Date Updated:
30/07/2008
Culture & Sensitivity - Aerobic (+ anaerobic if required)
Suitable Sample:
Swab, fluid and/or fresh tissue (indicate site on request form) Swab- anaerobic culture only if requested fluid and fresh tissue- anaerobic culture automatically.
(Air-dried smear for Gram stain)-optional
Unsuitable Sample:
Dry swabs Culture plates will not be accepted and will be disposed of if submitted.
Minimum Sample Size:
Turnaround Time:
Culture: 24-48 hours for most organisms (some take longer) Sensitivity: 48-72 hours for most organi
Request/Test Included in:
Request test
Comments:
Samples should be stored in a cool, dry place.
Samples for anaerobic culture should be received same day as collected & should not be refrigerated.
Samples stored at room temperature can be cultured for up to 3 days after collection
Samples stored at 4°C can cultured for up to 2 days after collection.
Any extra sensitivies must be requested within 24 hours of the results being sent out in order to get meaningful results
Extended incubation for up to 5d where no growth after 48 hours for all sterile sites.
Date Updated:
30/07/2008
Cyclosporin
Suitable Sample:
EDTA, needs to be fresh as possible
Unsuitable Sample:
SERUM LH
Minimum Sample Size:
4 mL
Turnaround Time:
Two (2) Working days
Request/Test Included in:
Request test
Comments:
Date Updated:
21/08/2008
Cytology - BAL (transtracheal)
Suitable Sample:
Sterile urine pot (can also collect some into EDTA if for cytology only)
Unsuitable Sample:
Minimum Sample Size:
1mL (small animals)
Turnaround Time:
24 hours Monday-Saturday
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Cytology - ear
Suitable Sample:
Air-dried smear(s) AND Swab
Unsuitable Sample:
Minimum Sample Size:
Single smear
Turnaround Time:
24 hours Monday-Saturday
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Cytology - eye
Suitable Sample:
Air-dried smear(s) AND Swab
Unsuitable Sample:
Minimum Sample Size:
Single smear
Turnaround Time:
24 hours Monday-Saturday
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Cytology - fluid/body cavity
Suitable Sample:
Fresh smears made at the time of collection are useful. EDTA is optimal for cytology Collect non-anticoagulated if sample is to be cultured; samples in EDTA are not suitable for culture
Unsuitable Sample:
LH
Minimum Sample Size:
0.75 mL if cell count is to be done Smears for cytology only
Turnaround Time:
24 hours Monday-Saturday
Request/Test Included in:
Request test
Comments:
If sufficient fluid, place some in an EDTA tube and the rest in either a SERUM tube (without activator) or a sterile urine container.
Date Updated:
30/07/2008
Cytology - FNA
Suitable Sample:
Air-dried smear(s)
Unsuitable Sample:
Minimum Sample Size:
Single smear
Turnaround Time:
24 hours Monday-Saturday
Request/Test Included in:
Request test
Comments:
Date Updated:
1/10/2007
Dexamethasone Suppresion Test (Equine) x3
Suitable Sample:
Three (3) x SERUM samples
Unsuitable Sample:
EDTA FL LH
Minimum Sample Size:
3 mL whole blood in each sample
Turnaround Time:
Daily
Request/Test Included in:
Request test
Comments:
Overnight DST protocol:
  • Collect serum sample for cortisol in the late afternoon (typically 5:00 pm)
  • Administer dexamethasone 40 ug / kg IM
  • Collect serum samples for cortisol 15 hours and 19 hours later (at 8:00 am and noon the next day)

  • Schott H.C. Pituitary pars intermedia dysfunction : Equine Cushing's Disease. Vet Clin Equine Practice 2002; 237-270.
    Date Updated:
    21/08/2008
    Dexamethasone Suppression Test (Equine) x2
    Suitable Sample:
    Two (2) x SERUM samples
    Unsuitable Sample:
    EDTA FL LH
    Minimum Sample Size:
    3 ml whole blood in each sample
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Overnight DST protocol( simplified method):
    1. Collect serum sample for cortisol in the late afternoon (typically 5:00 pm)
    2. Administer dexamethasone 40 ug / kg IM
    3. Collect serum sample for cortisol between 17 and 19 hours after administration of dexamethasone

    4. Schott H.C. Pituitary pars intermedia dysfunction : Equine Cushing's Disease. Vet Clin Equine Practice 2002; 237-270.
    Date Updated:
    22/08/2008
    Dexamethasone Suppression Test(LDDST)
    Suitable Sample:
    Three (3) x SERUM samples
    Unsuitable Sample:
    EDTA FL LH
    Minimum Sample Size:
    1.0 mL whole blood in each sample
    Turnaround Time:
    Daily
    Request/Test Included in:
    LDDST. Baseline (0 hour), four (4) hour and eight (8) hour samples - correctly labelled.
    Comments:
    A single cortisol is of minimal diagnostic value and dynamic tests of adrenal function are recommended for the diagnosis of both hypoadrenocorticism (Addisons) and hyperadrenocorticism (Cushings).
    HDDST generally no longer recommended.
    Date Updated:
    19/08/2008
    Digoxin
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH Gel separated clot activator (gel absorbs digoxin from sample)
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Two (2) working days
    Request/Test Included in:
    Request test
    Comments:
    Collect blood 8-10 hours post-dose (4-7 days after start or change in treatment). Do not use serum tubes that contain activator.
    Date Updated:
    1/10/2007
    Distemper Virus Antibody Titre by IFA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH CIT
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    CDV antibody titres by IFA
    Comments:
    Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations.Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.
    Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests.Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV.
    INTERPRETATION:
    Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
    Titres of >1:5 are considered seropositive.
    Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
    Dogs with titres of >1:20 probably do not require revaccination.
    Date Updated:
    1/10/2007
    Downer Cow Profile
    Suitable Sample:
    EDTA SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    FBC, Fibrinogen, Ca, Mg PO4, Urea, BHOB, AST, CPK
    Date Updated:
    30/06/2008
    Ear Cytology
    Suitable Sample:
    Air-dried smear(s) AND Swab
    Unsuitable Sample:
    Minimum Sample Size:
    Single smear
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Egg Count - Faecal
    Suitable Sample:
    Fresh Faeces
    Unsuitable Sample:
    Faecal swab
    Minimum Sample Size:
    2-3 gm (a larger volume such as 10 gm is better)
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    The saturated salt solution is efficient at floating common parasite eggs, but does not float trematode eggs and some tapeworm eggs. Specific procedures are required for these parasites.
    Faeces are stable for 3 days when refrigerated.
    Date Updated:
    1/10/2007
    Ehrlichia canis
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH
    Minimum Sample Size:
    2 - 3 mL whole blood
    Turnaround Time:
    Fourteen (14) Working days
    Request/Test Included in:
    Request test
    Comments:
    Test is run weekly and takes a week to complete.
    Date Updated:
    1/10/2007
    Electrolytes
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Na, Cl, K, HCO3, Ca, PO4
    Date Updated:
    7/07/2008
    Electrophoresis - Serum protein electrophoresis
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    One (1) day - Monday to Friday
    Request/Test Included in:
    Request Test
    Comments:
    Used primarily to differentiate polyclonal from monoclonal gammopathies.
    Date Updated:
    1/10/2007
    Equine Biochemistry
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    Na, K , Ca, Cl, HCO3, PO4, Urea, Creatinine, T.Protein, Alb, T.Bil, Alk Phos, GGT, AST, CPK.
    Date Updated:
    7/07/2008
    Equine Enzyme Profile
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    AST & CPK
    Date Updated:
    7/07/2008
    Equine Health and Fitness
    Suitable Sample:
    EDTA AND SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL EDTA AND 2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    FBC, Na,K, Ca,Cl, HCO3, PO4, Urea, Creatinine, T.Protein, Alb, TBil, Alk Phos, GGT, AST, CPK.
    Date Updated:
    30/06/2008
    Equine Health and Fitness + Fibrinogen
    Suitable Sample:
    EDTA AND SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL EDTA AND 2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    FBC, fibrinogen, Na, K, Ca, Cl, HCO3, PO4, Urea, Creatinine, T.Protein, Alb, T.Bil, Alk Phos, GGT, AST, CPK.
    Date Updated:
    30/06/2008
    Equine Herpesvirus (EHV1 and EHV4) Antibody Titres
    Suitable Sample:
    SERUM LH
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Eight (8) working days
    Request/Test Included in:
    Request test
    Comments:
    Please confirm price before sending
    Date Updated:
    7/07/2008
    Equine Herpesvirus Abortion(EHV-1 detection in tissue) PCR
    Suitable Sample:
    Lung and/or thymus from an aborted foetus
    Different tissues must be sent in separate container
    Do not use a fixing agent
    Refrigerate samples and send to the laboratory on ice.
    Frozen tissue acceptable.
    Unsuitable Sample:
    Fixed tissue
    Blood
    Minimum Sample Size:
    A piece of tissue approximately 1 x 1 x 1 cm is adequate
    Turnaround Time:
    Allow at least 10 working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Equine Infectious Anaemia (EIA) - Antibody Titre (Coggins Test)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH EDTA
    Minimum Sample Size:
    4 mL whole blood
    Turnaround Time:
    Three (3) working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Equine Viral Arteritis (EVA) Antibody Titre
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH EDTA
    Minimum Sample Size:
    4 mL whole blood
    Turnaround Time:
    Four (4) working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Eye Cytology
    Suitable Sample:
    Air-dried smear(s) AND Swab
    Unsuitable Sample:
    Minimum Sample Size:
    Single smear
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Factor VIII Clotting Factor
    Suitable Sample:
    CIT (1 part citrate to 9 parts blood) Fast 12 hours prior to collection, must be a resting sample.
    Unsuitable Sample:
    EDTA SERUM LH
    Minimum Sample Size:
    3 mL whole blood
    Turnaround Time:
    Are done as required and may take up to 7 working days.
    Request/Test Included in:
    Request test
    Comments:
    Beware of insufficiently filled OR overfilled CIT tubes and expired tubes.
    Separate and freeze the plasma ASAP
    If separating, do NOT transfer to tubes which contain clot activators (gel or powder)
    Plasma and whole blood can be kept chilled at 4°C for <24 hours.
    Plasma and whole blood can also be kep tat 20°C for <24 hours
    Frozen plasma is stable for <1 month and must remain frozen during transit
    Recommend prepare duplicate samples so one can be kept in reserve.
    It is not recommended that dogs be tested for vWD until six weeks after a significant bleeding episode.
    Please attach original citrate tube to the tube containing separated plasma on submission.
    Date Updated:
    1/10/2007
    Faecal Culture and Sensitivity
    Suitable Sample:
    Fresh faeces Faecal swab can be used, but is suboptimal. NOTE: If sample cannot be cultured immediately, store at room temperature prior to transport to the laboratory.
    Unsuitable Sample:
    Minimum Sample Size:
    2-3 gm (a larger volume such as 10 gm is better, especially for maintaining anaerobic conditions in the sample).
    Turnaround Time:
    12-24 hours for culture of routine organisms 48 hours for sensitivity on routine organisms
    Request/Test Included in:
    Request test
    Comments:
    Transport to the laboratory without refrigeration. At the laboratory samples are stored at 4oC to minimise overgrowth, but this will kill the more fragile organisms and poorer results will be obtained from samples stored before C&S is requested.
    Date Updated:
    1/10/2007
    Faecal Egg Count
    Suitable Sample:
    Fresh Faeces
    Unsuitable Sample:
    Faecal swab
    Minimum Sample Size:
    2-3 gm (a larger volume such as 10 gm is better)
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    The saturated salt solution is efficient at floating common parasite eggs, but does not float trematode eggs and some tapeworm eggs. Specific procedures are required for these parasites.
    Faeces are stable for 3 days when refrigerated.
    Date Updated:
    1/10/2007
    Faecal Fat, Starch and Fibre
    Suitable Sample:
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Request/Test Included in:
    Comments:
    Due to the low sensitivity and specificity of this testing it is no longer offered.
    Date Updated:
    02/02/2017
    Faecal Giardia and Cryptosporidium
    Suitable Sample:
    Fresh faeces The sample can be stored for up to 2 days at 2-8°C
    Unsuitable Sample:
    Blood
    Minimum Sample Size:
    1-2 gram (small volume)
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Detects Cryptosporidium parvum in animal faeces with reported sensitivity is 96.7% and specificity is 99.9%
    Detects Giardia duodenalsis antigen in dog and cat faeces with a reported sensitivity 97.2% and specificity 99.9%
    Date Updated:
    1/10/2007
    Faecal Multiplex Panel - Adult Equine
    Suitable Sample:
    Faeces in sterile container
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Equine (Adult) Faecal PCR Panel:

    - Lawsonia intracellularis
    - Clostridium difficile Toxin A/Toxin B
    - Clostridium perfringens Toxin A/CPE toxin/cpb2 toxin
    - Salmonella
    - Equine Coronavirus
    Date Updated:
    30/06/2016
    Faecal Multiplex Panel - Canine
    Suitable Sample:
    Faeces in sterile container
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Canine Faecal Multiplex PCR Panel:

    - BACTERIA
    Salmonella species
    Campylobacter jejuni
    - PARASITE
    Giardia species
    Cryptosporidium canis
    - VIRAL
    Parvovirus
    Coronavirus

    PCR testing of faecal samples allows evaluation for a range of infectious organisms in a single test – bacterial, protozoal and viral. For most infectious organisms included in the panel, PCR is the most sensitive and specific assay method. The PCR panel does not test for faecal parasites, and faecal parasite evaluation (faecal flotation) may be indicated in some cases.

    The sample required for PCR testing is a faecal sample collected into a sterile container.

    If other faecal tests are additionally required (including faecal float or culture), a separate faecal sample in a sterile container will need to be submitted.
    Date Updated:
    16/03/2017
    Faecal Multiplex Panel - Feline
    Suitable Sample:
    Faeces in sterile container
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Feline Faecal Multiplex PCR Panel:

    - BACTERIA
    Salmonella species
    Campylobacter jejuni
    - PARASITE
    Giardia species
    Cryptosporidium felis
    Toxoplasma gondii
    Tritrichomonas foetus
    - VIRAL
    Parvovirus
    Coronavirus

    PCR testing of faecal samples allows evaluation for a range of infectious organisms in a single test – bacterial, protozoal and viral. For most infectious organisms included in the panel, PCR is the most sensitive and specific assay method. The PCR panel does not test for faecal parasites, and faecal parasite evaluation (faecal flotation) may be indicated in some cases.

    The sample required for PCR testing is a faecal sample collected into a sterile container.

    If other faecal tests are additionally required (including faecal float or culture), a separate faecal sample in a sterile container will need to be submitted.
    Date Updated:
    30/06/2016
    Faecal Multiplex Panel - Foal
    Suitable Sample:
    Faeces in sterile container
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Equine (Foal) Faecal PCR Panel:

    - Clostridium difficile Toxin A/Toxin B
    - Clostridium perfringens Toxin A/CPE toxin/cpb2 toxin/netF toxin
    - Salmonella
    - Equine Coronavirus
    - Rotavirus
    - Cryptosporidium
    Date Updated:
    30/06/2016
    Faecal Occult Blood
    Suitable Sample:
    Fresh faeces (see Comments)
    Unsuitable Sample:
    Faecal swab
    Minimum Sample Size:
    2-3 gm (a larger volume such as 10 gm is better)
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Feed a non-red meat diet for 3 days before collection of faeces (white meat, cheese, rice, pasta).
    RBCs from anal bleeding and iron supplements will also interfere (false positive results).
    Date Updated:
    1/10/2007
    FBC
    Suitable Sample:
    EDTA CIT if <1 day old - can be done as a last option LH for some birds and reptiles
    Unsuitable Sample:
    SERUM
    Minimum Sample Size:
    1.0 mL, but prefer 2 mL
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    LH can be used for a CBC, but platelets and WBCs clump and the fibrinogen cannot be done.
    Date Updated:
    18/07/2008
    FBC - Avian
    Suitable Sample:
    Air-dried smear LH
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    FBC + Fibrinogen (Equine)
    Suitable Sample:
    EDTA
    Unsuitable Sample:
    SERUM
    Minimum Sample Size:
    Turnaround Time:
    Request/Test Included in:
    Comments:
    Date Updated:
    1/10/2007
    Feline Blood Group
    Suitable Sample:
    EDTA-preferred CIT-possible
    Unsuitable Sample:
    SERUM LH
    Minimum Sample Size:
    absolute minimum : 30- 40 uL
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    18/07/2008
    Feline Calicivirus Antibody Titre by IFA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH CIT
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Combined with feline herpes virus and panleukopenia virus titres
    Comments:
    This test is a screening test to determine the level of specific antiviral IgG in the serum. The titre is used as a measure of the humoral immunity. It has been shown that a titre of 1:5 on IFA is considered the appropriate levels to provide protective immunity. Note that it is possible that lower titres are protective and the influence of the cell mediated response cannot be reliably assessed in this manner.
    • Cats with a titre of <1:5 may not be adequately protected and should be revaccinated.
    • Cats with a titre of 1:5 but <1:20 should consider revaccination.
    • Cats with a titre of 1:20 or greater are considered protected and may not need revaccination. These animals should have their titre rechecked in approximately one year.
    Date Updated:
    27/04/2017
    Feline Haemotropic Mycoplasma PCR
    Suitable Sample:
    EDTA blood (dedicated sample, not for FBC as well)
    Unsuitable Sample:
    Serum, Lith Hep blood, Blood Films
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Feline Haemotropic Mycoplasma PCR:

    - Mycoplasma haemofelis
    - Mycoplasma haemominutum
    - Mycoplasma turicensis

    Haemotropic Mycoplasma cause significant disease in cats, and have been estimated to be present in 30% of cats with regenerative anaemia.

    Even in acute disease, haemotropic Mycoplasma are only identifiable by microscopy in freshly made smears of peripheral blood in 50% of cases. With exposure of blood samples to EDTA or following treatment, the percentage of blood film detection will drop dramatically. PCR provides a more sensitive method of detection of haemotropic Mycoplasma, with the additional advantage of differentiating the organisms involved.

    PCR testing also allows detection of carrier cats, which is particularly important for blood donors.

    The sample required for PCR testing is a blood sample collected into an EDTA blood tube.

    If a FBC is also required, a separate EDTA sample and blood smears need to be submitted.
    Date Updated:
    30/06/2016
    Feline Herpesvirus Antibody Titre by IFA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH CIT
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Combined feline calicivirus and panleukopenia virus titres
    Comments:
    This test is a screening test to determine the level of specific antiviral IgG in the serum. The titre is used as a measure of the humoral immunity. It has been shown that a titre of 1:5 on IFA is considered the appropriate levels to provide protective immunity. Note that it is possible that lower titres are protective and the influence of the cell mediated response cannot be reliably assessed in this manner.
    • Cats with a titre of <1:5 may not be adequately protected and should be revaccinated.
    • Cats with a titre of 1:5 but <1:20 should consider revaccination.
    • Cats with a titre of 1:20 or greater are considered protected and may not need revaccination. These animals should have their titre rechecked in approximately one year.
    Date Updated:
    27/04/2017
    Feline Immunodeficiency Virus (FIV) Antibody Detection
    Suitable Sample:
    EDTA SERUM LH
    Unsuitable Sample:
    FL
    Minimum Sample Size:
    0.5 mL whole blood
    Turnaround Time:
    Daily Monday-Friday
    Request/Test Included in:
    Request test Can be done in combination with FeLV soluble antigen ELISA
    Comments:
    Detects antibody.
    Cats exposed to FIV develop a detectable antibody response within 8 weeksof infection, but may be longer (<6 months).
    Once exposed, cats remain positive because infection with FIV is lifelong.
    Maternally-derived Abs affect results for < 6 months and kittens born to a positive or vaccinated queen may test positive without being infected; retest after 6 months of age.
    The test used by Vetnostics does NOT cross react with vaccinated cats.
    There is currently no reliable serology test available for detection of all strains of FIV virus, but this situation may change in the future.
    Any positive results should be confirmed with FIV PCR testing.
    Date Updated:
    27/04/2017
    Feline Leukaemia Virus (FeLV) Antigen Detection
    Suitable Sample:
    EDTA SERUM LH
    Unsuitable Sample:
    FL
    Minimum Sample Size:
    0.5 mL whole blood
    Turnaround Time:
    Daily Monday-Friday
    Request/Test Included in:
    Request test Can be done in combination with FIV antigen ELISA
    Comments:
    Date Updated:
    1/10/2007
    Feline Panleukopaenia Virus Antibody Titre by IFA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH CIT
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Combined with feline herpesvirus and calicivirus titres
    Comments:
    This test is a screening test to determine the level of specific antiviral IgG in the serum. The titre is used as a measure of the humoral immunity. It has been shown that a titre of 1:5 on IFA is considered the appropriate levels to provide protective immunity. Note that it is possible that lower titres are protective and the influence of the cell mediated response cannot be reliably assessed in this manner.
    • Cats with a titre of <1:5 may not be adequately protected and should be revaccinated.
    • Cats with a titre of 1:5 but <1:20 should consider revaccination.
    • Cats with a titre of 1:20 or greater are considered protected and may not need revaccination. These animals should have their titre rechecked in approximately one year.
    Date Updated:
    27/04/2017
    Feline Thyroid Profile
    Suitable Sample:
    EDTA SERUM LH
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL EDTA AND 2 mL whole blood
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    FBC(without diff, retic count), urea, creatinine, ALT, ALP, Ca, phosphate, plasma glucose, T4
    Date Updated:
    25/06/2008
    Feline Viral Studies (FIV/FeLV)
    Suitable Sample:
    EDTA SERUM LH
    Unsuitable Sample:
    FL
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Daily Monday-Friday
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Ferret Hormone Assay
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    0.5 mL
    Turnaround Time:
    Five (5) working days.
    Request/Test Included in:
    Request test
    Comments:
    Analysis of oestradiol and androstenedione
    Date Updated:
    15/03/2016
    Fibrinogen
    Suitable Sample:
    EDTA
    Unsuitable Sample:
    SERUM LH
    Minimum Sample Size:
    0.5 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Need specific test- heat precipitation (qualitative) in equine and production animals
    - instrument (quantitative) - dogs & cats
    Date Updated:
    7/07/2008
    FIP - Coronavirus Antibody Titre by IFA+
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH Species other than cats
    Minimum Sample Size:
    1.0 mL whole blood
    Turnaround Time:
    Four (4) working days.
    Request/Test Included in:
    Request test
    Comments:
    This is the gold-standard test for measurement of antibodies to feline coronavirus (FCoV).
    Titres of 1:10 or less are considered seronegative
    Titres 1:40 or greater considered seropositive
    Titres <1:10 indicate a low likelihood of coronavirus shedding. If the cat is infected they are likely to stop shedding when isolated.
    Cats with high titres (1:640 or higher) are almost always shedding virus but can stop shedding upon isolation. This can be demonstrated by a drop in their titre to low or undetectable levels.
    Cats with clinical FIP usually have a high titre (1:640 or higher) although a few cases may show low titres in the terminal stages (particularly in the effusive forms).
    The higher the titre the greater the specificity and sensitivity of this test for a diagnosis of FIP. The positive predictive index at 1:1600 is 0.94 and the negative predictive index 0.88 (J Vet Int Med 2003:17 781-790)
    No single test is suitable to confirm a diagnosis of FIP apart from histopathology. Clinical diagnosis of FIP should be based on a wide variety of tests including history, age, number of cats in household, persistent pyrexia and laboratory data including assessment of acute phase proteins, analysis of effusions, haematology and biochemical tests as well as specific serological tests to list a few.
    NOTE: This test can be done on feline or canine serum, but there are no guidelines for interpretation of canine results and it is not recommended for this species.
    Date Updated:
    1/10/2007
    FIP - Coronavirus direct IFA on effusion fluid
    Suitable Sample:
    effusion fluid- in EDTA tube(preferred) or plain tube for fresh samples.
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL fluid
    10 mL of fluid is preferable
    Turnaround Time:
    Two (2) Working days
    Request/Test Included in:
    Request test
    Comments:
    This is a highly specific test to demonstrate FIP-related antigens in macrophages in effusion fluid.
    The anti-FCoV direct IFA test detects direct immunofluorescent staining of IP-related antigens in macrophages in the effusion with anti-FCoV antibodies. This is a very specific diagnostic test as only FIP-infected cats will have virus-infected macrophages. This test has a specificity of 1.00 (100% positive predictive value) but a relatively low sensitivity of 0.57.
    No single test is able to confirm a diagnosis of FIP apart from histopathology. Clinical diagnosis of FIP should be based on a wide variety of tests including history, age, number of cats in household, persistent pyrexia and laboratory data including assessment of acute phase proteins, analysis of effusions, haematology and biochemical tests as well as specific serological tests to list a few.
    A fluid analysis is required of the effusion prior to this test being performed.
    Date Updated:
    25/07/2008
    FIV PCR Assay
    Suitable Sample:
    EDTA
    Unsuitable Sample:
    Minimum Sample Size:
    0.5ml to 1ml blood
    Turnaround Time:
    5-6 working days
    Request/Test Included in:
    Comments:
    To differentiate between FIV vaccination and natural infection in cats
    Date Updated:
    7/07/2008
    Fluid Analysis and Cytology (eg peritoneal, pleural fluids)
    Suitable Sample:
    EDTA
    AND
    smears from site
    Unsuitable Sample:
    Minimum Sample Size:
    1-2 mL
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Fluoroacetate (1080)
    Suitable Sample:
    Urine
    Stomach Contents
    Unsuitable Sample:
    EDTA
    SERUM
    Minimum Sample Size:
    Turnaround Time:
    Up to forty five (45) days
    Request/Test Included in:
    Request test
    Comments:
    Preferably send samples frozen.
    Date Updated:
    7/07/2008
    FNA
    Suitable Sample:
    Air-dried smear(s)
    Unsuitable Sample:
    Minimum Sample Size:
    Single smear
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Foal IgG - TIA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    This rapid quantitative test has replaced both the glutaraldehyde test and RID. It is used for both screening passive transfer and insurance purposes.
    Date Updated:
    21/11/2016
    Foal Profile
    Suitable Sample:
    SERUM
    EDTA
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL EDTA
    AND
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    FBC, Fibrinogen, Na, K, Cl, HCO3, Ca, PO4, Urea, Creatinine, Glucose.
    Date Updated:
    30/06/2008
    Folate
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    1.5 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Free T4 by Equilibrium Dialysis
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Up to fifteen (15) days
    Request/Test Included in:
    Request test
    Comments:
    Not recommended as initial test - follow up to confirm canine hypothyroidism
    Date Updated:
    21/11/2016
    Fructosamine
    Suitable Sample:
    EDTA
    SERUM
    LH
    Unsuitable Sample:
    FL
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Is an indication of blood glucose over the previous 2-3 weeks and can be used to either distinguish between transient and persistent hyperglycaemia, or to monitor diabetic patients.
    Date Updated:
    1/10/2007
    Fungal Culture
    Suitable Sample:
    Hairs
    Tissue
    Unsuitable Sample:
    Minimum Sample Size:
    Very small samples are less likely to yield a positive culture.
    Turnaround Time:
    1 to 4 weeks depending on when/if fungus grows
    Request/Test Included in:
    Request test
    Comments:
    Cultures are examined weekly for up to 4 weeks.
    Date Updated:
    1/10/2007
    Gammaglutamyl transferase (GGT)
    Suitable Sample:
    SERUM
    EDTA
    Unsuitable Sample:
    LH
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Haemolysis and the presence of RBCs in the plasma/serum will increase the value.
    Calves and lambs have elevated GGT values due to colostral transfer.
    GGT cannot be used as a measure of colostral transfer in foals, but foals may have a transient increase in GGT in the first 3 weeks of life
    GGT cannot be used as a measure of colostral transfer in cria
    Date Updated:
    1/10/2007
    Gentamicin
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Gentamicin x 2
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    GGT
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Giardia Antigen in Faeces (Dogs and Cats)
    Suitable Sample:
    Fresh faeces
    The sample can be stored for up to 2 days at 2-8°C
    Unsuitable Sample:
    Blood
    Minimum Sample Size:
    1-2 gram (small volume)
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Detects Cryptosporidium parvum in animal faeces with reported sensitivity is 96.7% and specificity is 99.9%
    Detects Giardia duodenalsis antigen in dog and cat faeces with a reported sensitivity 97.2% and specificity 99.9%
    Date Updated:
    1/10/2007
    GLDH (glutamate dehydrogenase)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Globulins
    Suitable Sample:
    SERUM
    LH
    EDTA
    Unsuitable Sample:
    Minimum Sample Size:
    2mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Glucose
    Suitable Sample:
    FL is the best sample
    SERUM, LH and EDTA can be used but will give lower results if the plasma has not been removed from the cells soon after collection
    Unsuitable Sample:
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Samples collected in FL-EDTA give an accurate assessment of glucose at the time of collection. Glucose in other samples will decrease steadily after collection as the cells continue to consume it. Serum samples will often give very low glucose results.
    Date Updated:
    1/10/2007
    Glutathione Peroxidase (Selenium)
    Suitable Sample:
    LH
    Unsuitable Sample:
    SERUM
    EDTA
    Minimum Sample Size:
    10 mL whole blood
    Turnaround Time:
    Eight (8) working days
    Request/Test Included in:
    Request test
    Comments:
    Glutathione peroxidase (GPx) is used to assess selenium status.
    Batch discount available.
    Date Updated:
    2/07/2008
    Greyhound Profile
    Suitable Sample:
    SERUM
    EDTA
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL EDTA
    AND
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    FBC, Alb, urea,creatinine, Na,K,Cl,Ca, Phosphate,ALT,ALP,AST,CPK.
    Date Updated:
    25/06/2008
    Haemobartonella felis (Mycoplasma haemofelis) - smear
    Suitable Sample:
    EDTA
    Unsuitable Sample:
    Minimum Sample Size:
    1 smear
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Heartworm Antigen (including for export)
    Suitable Sample:
    SERUM
    EDTA
    LH
    Unsuitable Sample:
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Can be used in dogs and cats, but for cats, screen first with a heartworm antibody test.
    Can also be used in ferrets.
    Date Updated:
    1/10/2007
    Heartworm Concentration for Microfilaria
    Suitable Sample:
    EDTA
    LH
    Unsuitable Sample:
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Herpesvirus (Feline) Antibody Titre by IFA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    CIT
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Individual test
    Combined feline herpesvirus, calicivirus and panleukopenia
    Comments:
    This test is a screening test to determine the level of specific antiviral IgG in the serum. The titre is used as a measure of the humoral immunity. It has been shown that a titre of 1:5 on IFA is considered the appropriate levels to provide protective immunity. Note that it is possible that lower titres are protective and the influence of the cell mediated response cannot be reliably assessed in this manner.
    • Cats with a titre of <1:5 may not be adequately protected and should be
      revaccinated.
    • Cats with a titre of 1:5 but <1:20 should consider revaccination.
    • Cats with a titre of 1:20 or greater are considered protected and may not
      need revaccination. These animals should have their titre rechecked in
      approximately one year.
    Date Updated:
    1/10/2007
    Herpesvirus (Feline) Detection by PCR
    Suitable Sample:
    Swab of tears or exudate (see Comments)
    Unsuitable Sample:
    Sample in transport medium.
    Frozen sample.
    Minimum Sample Size:
    Single swab.
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Feline respiratory profile by PCR
    Comments:
    Use a dry sterile cotton swab to collect the sample.
    Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs or the appropriate lesion.
    Place the swab in a sterile container and refrigerate.
    Do not freeze.
    Do not place in transport medium.
    Date Updated:
    1/10/2007
    Histopathology - 1 specimen
    Suitable Sample:
    Tissue in 10x volume of buffered formalin
    Unsuitable Sample:
    Tissue in any other fluid
    Minimum Sample Size:
    Tissue in 10x volume of buffered formalin
    Turnaround Time:
    Two (2) - Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Histopathology - 2 specimens
    Suitable Sample:
    Tissues in 10x volume of buffered formalin
    Unsuitable Sample:
    Tissues in any other fluid
    Minimum Sample Size:
    Tissues in 10x volume of buffered formalin
    Turnaround Time:
    Two (2) - Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Histopathology - 3 specimens
    Suitable Sample:
    Tissues in 10x volume of buffered formalin
    Unsuitable Sample:
    Tissues in any other fluid
    Minimum Sample Size:
    Tissues in 10x volume of buffered formalin
    Turnaround Time:
    Two (2) - Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Histopathology - 4 specimens
    Suitable Sample:
    Tissues in 10x volume of buffered formalin
    Unsuitable Sample:
    Tissues in any other fluid
    Minimum Sample Size:
    Tissues in 10x volume of buffered formalin
    Turnaround Time:
    Two (2) - Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Histopathology - Dermata Specialist
    Suitable Sample:
    Tissues in 10x volume of buffered formalin
    Unsuitable Sample:
    Tissues in any other fluid
    Minimum Sample Size:
    Tissues in 10x volume of buffered formalin
    Turnaround Time:
    Five (5) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Histopathology - Multiorgan
    Suitable Sample:
    Tissues in 10x volume of buffered formalin
    Unsuitable Sample:
    Tissues in any other fluid
    Minimum Sample Size:
    Tissues in 10x volume of buffered formalin
    Turnaround Time:
    Two (2) - Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Histopathology - Processing Only
    Suitable Sample:
    Tissues in 10x volume of buffered formalin
    Unsuitable Sample:
    Tissues in any other fluid
    Minimum Sample Size:
    Tissues in 10x volume of buffered formalin
    Turnaround Time:
    One (1) Working days
    Request/Test Included in:
    Request test
    Comments:
    For contract work only.
    Date Updated:
    1/10/2007
    Immunoglobulins - Cria (RID)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Two (2) - Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    For use in camelid species (alpaca, llama)
    Date Updated:
    1/10/2007
    Immunoglobulins - Foal (Glutaraldehyde coagulation test, GCT)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    This is a useful screening test for failure of passive transport in foals.
    It is also useful in calves
    This test is not validated for camelids; for these species a specific RID test must be done.
    Date Updated:
    1/10/2007
    Immunoglobulins - Foal (RID)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Two (2) working days
    Request/Test Included in:
    Request test
    Comments:
    This is the screening test for failure of passive transfer in foals at 24 hours.
    Date Updated:
    1/10/2007
    Immunohistochemistry - FIP
    Suitable Sample:
    Tissue in 10x volume of buffered formalin
    Unsuitable Sample:
    SERUM
    EDTA
    CIT
    Minimum Sample Size:
    Tissue in 10x volume of buffered formalin
    Turnaround Time:
    Five (5) Working days following routine histopathology
    Request/Test Included in:
    Request test
    Comments:
    This is a highly specific test to demonstrate FIP-related antigens in macrophages.
    The anti-FCoV direct IFA test detects direct immunofluorescent staining of IP-related antigens in macrophages in the tissue. This is a very specific diagnostic test as only FIP-infected cats will have virus-infected macrophages. This test has a specificity of 1.00 (100% positive predictive value) but a relatively low sensitivity of 0.57.
    No single test is able to confirm a diagnosis of FIP apart from histopathology.
    Date Updated:
    1/10/2007
    Immunohistochemistry - other stains
    Suitable Sample:
    Tissue in 10x volume of buffered formalin
    Unsuitable Sample:
    SERUM
    EDTA
    CIT
    Minimum Sample Size:
    Tissue in 10x volume of buffered formalin
    Turnaround Time:
    Five (5) Working days following routine histopathology
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Immunohistochemistry - T and B cell
    Suitable Sample:
    Tissue in 10x volume of buffered formalin
    Unsuitable Sample:
    SERUM
    EDTA
    CIT
    Minimum Sample Size:
    Tissue in 10x volume of buffered formalin
    Turnaround Time:
    Three (3) Working days following routine histopathology
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Immunohistochemistry - T cell
    Suitable Sample:
    Tissue in 10x volume of buffered formalin
    Unsuitable Sample:
    SERUM
    EDTA
    CIT
    Minimum Sample Size:
    Tissue in 10x volume of buffered formalin
    Turnaround Time:
    Three (3) Working days following routine histopathology
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Inhibin
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    6-10 mL whole blood
    Turnaround Time:
    Eight (8) Working days
    Request/Test Included in:
    Request test
    Comments:
    Increased in 87.2% of mares with granulosa cell tumours to >0.54 +/- 0.06 ng/mL (CCVE 1997).
    Date Updated:
    1/10/2007
    Insulin
    Suitable Sample:
    SERUM
    LH
    Sodium fluoride can be used but will give slightly lower values
    Unsuitable Sample:
    EDTA (negative interference)
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    If suspect insulinoma, collect FL sample for concurrent glucose.
    Date Updated:
    1/10/2007
    Iron (serum iron)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Johnne's Disease
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    4 mL whole blood (into tube treated for clot retraction)
    Turnaround Time:
    10 working days
    Request/Test Included in:
    Request test
    Comments:
    Complement fixation
    OR
    ELISA
    Date Updated:
    1/10/2007
    Johnne's Disease - Batch
    Suitable Sample:
    Faeces
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Joint fluid cytology
    Suitable Sample:
    Fresh smears made at the time of collection are useful.
    EDTA is optimal for cytology
    Collect non-anticoagulated if sample is to be cultured; samples in EDTA are not suitable for culture
    Unsuitable Sample:
    LH
    Minimum Sample Size:
    0.5 mL if cell count is to be done
    Smears for cytology only
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    If sufficient fluid, place some in an EDTA tube and the rest in either a SERUM tube (without activator) or a sterile urine container.
    Date Updated:
    1/10/2007
    Ketones (Beta-hydroxybutyrate (BOHB)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    If the glucose is elevated in a profile, the BOHB is automatically included.
    Comments:
    Date Updated:
    27/08/2008
    Kidney Profile
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    T.Protein, alb, urea, creatinine, Ca, Phosphate, K.
    Date Updated:
    26/06/2008
    Koala - Chlamydia by PCR+
    Suitable Sample:
    Conjunctival swab
    AND/OR
    Cloacal swab
    See below for sample collection
    Unsuitable Sample:
    Blood
    Swabs in transport medium
    Minimum Sample Size:
    One swab
    If more than one swab is submitted per animal, each swab is charged for separately
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Chlamydia PCR for koalas
    Comments:
    Use a dry sterile cotton swab to collect the sample.
    Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs and/or cloaca.
    Place the swab in a sterile container and refrigerate.
    Use a separate container for each animal if collecting from more than one animal.
    Do not freeze.
    Do not place in transport medium.
    Date Updated:
    1/10/2007
    LDDST (Low Dose Dexamethasone Suppression Test)+
    Suitable Sample:
    Three (3) x SERUM samples
    Unsuitable Sample:
    EDTA
    FL
    LH
    Minimum Sample Size:
    1.0 mL whole blood in each sample
    Turnaround Time:
    Daily
    Request/Test Included in:
    LDDST. Baseline (0 hour), four (4) hour and eight (8) hour samples - correctly labelled
    Comments:
    A single cortisol is of minimal diagnostic value and dynamic tests of adrenal function are recommended for the diagnosis of both hypoadrenocorticism (Addisons) and hyperadrenocorticism (Cushings).
    HDDST generally no longer recommended.
    Date Updated:
    19/08/2008
    Lead (blood levels)
    Suitable Sample:
    EDTA

    LH
    Unsuitable Sample:
    SERUM
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Monday, Wednesday and Friday
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    30/07/2008
    Leishmania spp IFAT serology
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Run on Monday and Thursday
    Cutoff time 9:00 am at Vetpath
    Results available later the same d
    Request/Test Included in:
    Request test
    Comments:
    If the sample is going to be in transit for more than a day, it is recommended that the serum is separated off if possible and the serum rather than whole is sent (this minimises haemolysis)
    Date Updated:
    1/10/2007
    Leptospira Antibody Titre(s)
    Suitable Sample:
    SERUM (clear serum, no particles or fibrin tags)
    Unsuitable Sample:
    LH
    EDTA
    Minimum Sample Size:
    4 mL whole blood
    Turnaround Time:
    Three (3) working days
    Request/Test Included in:
    Request test
    Comments:
    For the dog, horse - reference MAT panel incorporates 22 serovars tested at the WHO leptospirosis reference lab.
    All positive results are titred out.
    If the test is being done on a dog for export, verify what level the test is to be titred to (the requirement will differ from country to country)
    The test for Leptospira canicola (for canine export) is a MAT
    Date Updated:
    29/07/2008
    Leptospira PCR (urine/blood)
    Suitable Sample:
    Urine or EDTA blood
    Unsuitable Sample:
    Serum, Lith Hep blood
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Canine Leptospira PCR: - Leptospira interrogans PCR detection of leptospirosis allows detection of infection earlier than serology. Dogs take at least a week after exposure to become serologically positive. However, they will be PCR positive in blood within the first week. The preferred sample within the first week of infection is blood. After this time, the blood will commonly become negative, and urine is the preferred sample. However, as the time of infection is rarely known, both blood and urine should be submitted in all suspected cases. When both samples are submitted at the same time, both will be tested at no additional cost. Samples for PCR should be collected before starting antibiotic therapy. The samples required for PCR testing are urine in a sterile urine container and a blood sample collected into an EDTA blood tube. If haematology, biochemistry and urinalysis are also required for the case, separate blood and urine samples need to be submitted.
    Date Updated:
    30/06/2016
    Lipase
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Liver Fluke - ELISA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    4 mL whole blood
    Turnaround Time:
    Three (3) working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Liver Fluke (detection of eggs in faeces)
    Suitable Sample:
    Faeces
    Unsuitable Sample:
    Blood
    Minimum Sample Size:
    10 gm faeces
    Turnaround Time:
    Three (3) working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Liver Profile - Large
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    T.Bil(conj. if necessary), T.Protein, Alb, Glob, AST, GLDH, GGT, ALP.
    Date Updated:
    26/06/2008
    Liver Profile - Small
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    T.Protein, Alb, T.Bil, ALP, GGT, ALT
    Date Updated:
    26/06/2008
    Low Dose Dexamethasone Suppression Test (LDDST, cortisol x 3)+
    Suitable Sample:
    Three (3) x SERUM samples
    Unsuitable Sample:
    EDTA
    FL
    LH
    Minimum Sample Size:
    1.0 mL whole blood in each sample
    Turnaround Time:
    Daily
    Request/Test Included in:
    LDDST. Baseline (0 hour), four (4) hour and eight (8) hour samples - correctly labelled
    Comments:
    A single cortisol is of minimal diagnostic value and dynamic tests of adrenal function are recommended for the diagnosis of both hypoadrenocorticism (Addisons) and hyperadrenocorticism (Cushings).
    HDDST generally no longer recommended
    Date Updated:
    19/08/2008
    Magnesium (Mg)
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    FL
    CIT
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Mare Pregnancy (MIP) 40-120days
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Five(5) Days
    Request/Test Included in:
    Request test
    Comments:
    Measures PMSG(Pregnant Mare Serum Gonadotrophin) produced by endometrial cups from 40-120 days of gestation.
    Oestrone sulphate should be used to confirm pregnancy and the presence of a live foal after 100 days
    Date Updated:
    29/07/2008
    Mare Pregnancy (oestrone sulphate) 80+ days
    Suitable Sample:
    SERUM
    EDTA
    Unsuitable Sample:
    Haemolysed samples
    Lipaemic samples
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Seven (7) days
    Request/Test Included in:
    Request test
    Comments:
    Samples should be separated and the serum/plasma stored in the freezer once it reaches Vetnostics.
    Oestrone (estrone) sulphate is a steroid hormone detectable in the circulation in many species. It can be produced in the gonads, the adrenals and is produced by the foetal-placental unit during pregnancy.
    Pregnancy >80 days: Oestrone sulphate levels rise during pregnancy and are a good indicator of a viable pregnancy. In mares oestrone sulphate is detectable in the maternal circulation from early as 60 days post-mating until foaling. In the event of foetal death oestrone sulphate disappears rapidly from circulation.
    Cryptorchidism in horses 3yo or older.
    Goat pregnancy >50 days post-service
    Date Updated:
    1/10/2007
    Masticatory myositis - 2M antibody
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    CIT
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Fifiteen (15) days
    Request/Test Included in:
    Request test
    Comments:
    Diagnostic test for masticatory muscle myositis in dogs
    False negative results may occur if the dog has been on immunosuppressive doses of corticosteroids for 7-10 days.
    Date Updated:
    1/10/2007
    McMaster Egg Count
    Suitable Sample:
    Fresh Faeces
    Unsuitable Sample:
    Faecal swab
    Minimum Sample Size:
    2-3 gm (a larger volume such as 10 gm is better)
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    The saturated salt solution is efficient at floating common parasite eggs, but does not float trematode eggs and some tapeworm eggs. Specific procedures are required for these parasites.
    Faeces are stable for 3 days when refrigerated.
    Date Updated:
    1/10/2007
    Microfilaria
    Suitable Sample:
    EDTA
    LH
    Unsuitable Sample:
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Examines for both Dipetolenema and Dirofilaria
    Date Updated:
    7/07/2008
    Milk Swab (>5)
    Suitable Sample:
    Fresh milk samples (may be frozen)
    Unsuitable Sample:
    Swab
    Minimum Sample Size:
    1 - 2 mL
    Turnaround Time:
    Two (2) working days
    Request/Test Included in:
    Request test
    Comments:
    Frozen samples are stable for < 7 days (the fat protects the organisms)
    Date Updated:
    1/10/2007
    Milk Swab (2-3)
    Suitable Sample:
    Fresh milk samples (may be frozen)
    Unsuitable Sample:
    Swab
    Minimum Sample Size:
    1 - 2 mL
    Turnaround Time:
    Two (2) working days
    Request/Test Included in:
    Request test
    Comments:
    Frozen samples are stable for < 7 days (the fat protects the organisms)
    Date Updated:
    1/10/2007
    Milk Swab (4-5)
    Suitable Sample:
    Fresh milk samples (may be frozen)
    Unsuitable Sample:
    Swab
    Minimum Sample Size:
    1 - 2 mL
    Turnaround Time:
    Two (2) working days
    Request/Test Included in:
    Request test
    Comments:
    Frozen samples are stable for < 7 days (the fat protects the organisms)
    Date Updated:
    1/10/2007
    Myasthenia gravis (AChR Ab)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    CIT
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Ten (10) working days (2 weeks)
    Request/Test Included in:
    Request test
    Comments:
    Diagnostic test for myasthenia gravis
    Date Updated:
    1/10/2007
    Mycoplasma hemofelis by PCR
    Suitable Sample:
    EDTA
    Unsuitable Sample:
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Five (5) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Neospora IgG Antibody Titre by IFA (canine and feline)
    Suitable Sample:
    SERUM (must specify if canine or feline)
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    1.5 mL whole blood (0.5 mL clear serum)
    Turnaround Time:
    Five (5) working days
    Request/Test Included in:
    Request test
    Comments:
    The test is species-specific (canine and feline available)
    This test considers a titre of 1:16 to be seropositive.
    Seropositivity indicates exposure to antigens and does not imply clinical disease. Note that parasitologically confirmed cases of neosporosis were shown to have titres of 1:200 or above and clinical cases may have titres in excess of 1:1200.
    Reference:Vet Parasitol (1996) 67:1-59.
    Date Updated:
    1/10/2007
    Occult blood - faeces
    Suitable Sample:
    Fresh faeces (see Comments)
    Unsuitable Sample:
    Faecal swab
    Minimum Sample Size:
    2-3 gm
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Feed a non-red meat diet for 3 days before collection of faeces (white meat, cheese, rice, pasta).
    RBCs from anal bleeding and iron supplements will also interfere (false positive results).
    Date Updated:
    1/10/2007
    Oestradiol
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Three (3) days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Oestrone Sulphate ELISA (quantitative)
    Suitable Sample:
    SERUM
    EDTA
    Unsuitable Sample:
    Haemolysed samples
    Lipaemic samples
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Seven (7) days
    Request/Test Included in:
    Request test
    Comments:
    Samples should be separated and the serum/plasma stored in the freezer once it reaches Vetnostics.
    Oestrone (estrone) sulphate is a steroid hormone detectable in the circulation in many species. It can be produced in the gonads, the adrenals and is produced by the foetal-placental unit during pregnancy.
    Pregnancy >80 days: Oestrone sulphate levels rise during pregnancy and are a good indicator of a viable pregnancy. In mares oestrone sulphate is detectable in the maternal circulation from early as 60 days post-mating until foaling. In the event of foetal death oestrone sulphate disappears rapidly from circulation.
    Cryptorchidism in horses 3 yo or older.
    Goat pregnancy >50 days post-service
    Date Updated:
    1/10/2007
    Oestrus Cytology (Vaginal cytology)
    Suitable Sample:
    Air-dried smear(s)
    Swab
    Unsuitable Sample:
    Minimum Sample Size:
    Single smear
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    May be used in conjunction with serum progesterone.
    Date Updated:
    1/10/2007
    Organophosphates
    Suitable Sample:
    Liver
    Stomach Contents
    Unsuitable Sample:
    EDTA
    SERUM
    Minimum Sample Size:
    >50 mL stomach contents
    Turnaround Time:
    Up to forty five (45) days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Pancreatic Profile
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Na, K, Ca, Cl, HCO3, PO4, Urea, Creatinine, T.Protein,Alb, Glob,TBil, Alk Phos, ALT, GGT, AST, CPK, Glucose, Trig, cholesterol,(BHOB if necessary), Amylase, Lipase
    Date Updated:
    30/06/2008
    Pancreatic Profile + FBC
    Suitable Sample:
    EDTA
    AND
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL EDTA
    AND
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    Date Updated:
    1/10/2007
    Panleukopaenia (feline) Antibody Titre by IFA+
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    CIT
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Individual test
    Combined feline herpesvirus, calicivirus and panleukopenia
    Comments:
    This test is a screening test to determine the level of specific antiviral IgG in the serum. The titre is used as a measure of the humoral immunity. It has been shown that a titre of 1:5 on IFA is considered the appropriate levels to provide protective immunity. Note that it is possible that lower titres are protective and the influence of the cell mediated response cannot be reliably assessed in this manner.
    • Cats with a titre of <1:5 may not be adequately protected and should be
      revaccinated.
    • Cats with a titre of 1:5 but <1:20 should consider revaccination.
    • Cats with a titre of 1:20 or greater are considered protected and may not
      need revaccination. These animals should have their titre rechecked in
      approximately one year.
    Date Updated:
    1/10/2007
    Parathyroid hormone (PTH)
    Suitable Sample:
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Request/Test Included in:
    Comments:
    From October 2016 this test will no longer be available. We are evaluating the validity of alternative test methods and will notify clients when a suitable alternative is available.
    Date Updated:
    1/11/2016
    Parathyroid-related protein (PTHrp)
    Suitable Sample:
    EDTA tube with 200 uL Trasylol (see comments)
    Unsuitable Sample:
    SERUM
    LH
    SC
    Minimum Sample Size:
    At least 2.0 mL whole blood
    Turnaround Time:
    Seven (7) working days.
    Request/Test Included in:
    Request Test, contact laboratory in advance.
    Comments:
    This is NOT the same as PTH
    To collect the sample, hospitalise, fast overnight and collect 2 - 5 ml blood into a chilled plastic EDTA tube with 200 uL Trasylol.
    The sample must be centrifuged immediately and the plasma transferred to a chilled plain tube.
    If sufficient volume, split the sample and keep a frozen duplicate plasma at the clinic.
    Freeze ASAP, plasma must not thaw! Freeze overnight before sending in.
    Date Updated:
    1/10/2007
    Parvovirus Antibody Titre by IFA(Canine)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA LH
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    10 working days
    Request/Test Included in:
    CPV antibody titre by IFA.
    Combined CDV & CPA IFA titres can also be requested.
    Comments:
    Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations. Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease. Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests. Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV. INTERPRETATION: Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended. Titres of >1:5 are considered seropositive. Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased. Dogs with titres of >1:20 probably do not require revaccination.
    Date Updated:
    24/.3/2016
    Parvovirus Antigen (Faeces)
    Suitable Sample:
    Faeces
    Faecal swab
    Unsuitable Sample:
    Blood
    Minimum Sample Size:
    Faecal swab
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    False negative results may occur as the period of viral shedding in the faeces is relatively brief and virus is seldom detected 10 to 12 days after infection (this corresponds to 5 to 7 days of clinical illness).
    False negatives may also occur very early in infection; retest in 36 to 48 hours if this is suspected.
    Modified live vaccination results in transient faecal shedding and may give a weak positive result 5-15 days after vaccination.
    Date Updated:
    1/10/2007
    Peritoneal fluid analysis and cytology
    Suitable Sample:
    EDTA
    AND
    smears from site
    Unsuitable Sample:
    Minimum Sample Size:
    1-2 mL
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Pestivirus Antigen ELISA
    Suitable Sample:
    LH
    EDTA
    Serum but not in SST tube
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Five (5) working days.
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Phenobarbitone
    Suitable Sample:
    SERUM (without serum separator gel)
    LH
    EDTA
    Unsuitable Sample:
    Minimum Sample Size:
    2 ml whole blood
    Turnaround Time:
    2 hours
    Request/Test Included in:
    Request test
    Comments:
    Collect sample at any time between doses.
    Thiopentone anaesthesia does not interfere.
    Date Updated:
    1/10/2007
    Phenytoin
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 ml whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Phosphate (inorganic)
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    CIT
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Haemolysis will increase the value measured
    Lipaemia will increase the value measured
    Date Updated:
    1/10/2007
    Platelet Count
    Suitable Sample:
    EDTA
    CIT
    Unsuitable Sample:
    SERUM
    LH
    Minimum Sample Size:
    1.0 mL, but prefer 2 mL
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Pleural fluid analysis and cytology
    Suitable Sample:
    EDTA
    AND
    smears from site
    Unsuitable Sample:
    Minimum Sample Size:
    1-2 mL
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    PMSG (Pregnant Mare Serum Gonadotrophin)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Five (5) Days
    Request/Test Included in:
    Request test
    Comments:
    Measures PMSG(Pregnant Mare Serum Gonadotrophin) produced by endometrial cups from 40-120 days of gestation.
    Oestrone sulphate should be used to confirm pregnancy and the presence of a live foal after 100 days
    Date Updated:
    29/07/2008
    Poisoning - 1080
    Suitable Sample:
    Stomach Contents
    Urine
    Unsuitable Sample:
    EDTA
    SERUM
    Minimum Sample Size:
    Turnaround Time:
    Up to forty five (45) days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    26/07/2008
    Poisoning - General
    Suitable Sample:
    Urine
    Stomach Contents
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Three (3) working days.
    Request/Test Included in:
    Request test
    Comments:
    Includes Opiates, Sympathetic Amines, Benzodiazepines, Cannabinoids, Barbiturates, Cocaine metabolites
    Includes strychnine but not 1080
    Date Updated:
    26/07/2008
    Poisoning - OP
    Suitable Sample:
    Liver
    Stomach Contents
    Unsuitable Sample:
    EDTA
    SERUM
    Minimum Sample Size:
    >50 mL stomach contents
    Turnaround Time:
    Up to forty five (45) days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Poisoning - Rodenticide
    Suitable Sample:
    For postmortem samples liver is the best sample (preferably frozen)
    Vomitus - preferably frozen
    Unsuitable Sample:
    Blood
    Urine
    Minimum Sample Size:
    10 gm minimum, but the more the better
    Turnaround Time:
    Allow <6 weeks (contact laboratory to confirm expected time for availability of results).
    Request/Test Included in:
    Request test
    Comments:
    Quantitative assays for brodifacoum, bromodiolone, warfarin, coumatetrallyl and pindone
    Date Updated:
    1/10/2007
    Polyoma Virus (Avian) by PCR
    Suitable Sample:
    LH blood
    Feathers
    Tissue (liver or spleen)
    Droppings OR cloacal swab
    Unsuitable Sample:
    EDTA
    SERUM
    Minimum Sample Size:
    Blood: volume of LH blood depends on size of bird, a few drops from smaller species is acceptable
    Feathers: at least 3 freshly plucked feathers (send in small plastic bag or container)
    Turnaround Time:
    Approximately 2 weeks (confirm with laboratory)
    Request/Test Included in:
    Request test
    Comments:
    Also called budgerigar fledgling virus (but occurs in all psittacine birds).
    PCR test
    Date Updated:
    1/10/2007
    Potassium (K)
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    CIT
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    LH decreases the problem of leakage of K from platelets during transit
    Haemolysis will increase the value in some species
    Date Updated:
    1/10/2007
    Potassium bromide
    Suitable Sample:
    SERUM (without serum separator gel)
    LH
    Unsuitable Sample:
    EDTA
    CIT
    FL
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Two (2) - Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    Collect sample 1 hour prior to the next dose of KBr
    Mild lipaemia and/or haemolysis is acceptable.
    Date Updated:
    1/10/2007
    Pre-Anaesthetic Profile
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Pregnancy - Bitch (Relaxin)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA CIT LH FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Sample should be collected at 30 days + this test can be used in the cat
    Date Updated:
    7/07/2008
    Pregnancy - Mare (<80 days) PMSG
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Five(5) Days
    Request/Test Included in:
    Request test
    Comments:
    Measures PMSG(Pregnant Mare Serum Gonadotrophin) produced by endometrial cups from 40-120 days of gestation. Oestrone sulphate should be used to confirm pregnancy and the presence of a live foal after 100 days.
    Date Updated:
    29/07/2008
    Pregnancy - Mare (>80 days) oestrone sulphate ELISA (quantitative)
    Suitable Sample:
    SERUM EDTA
    Unsuitable Sample:
    Haemolysed samples Lipaemic samples
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Seven (7) days
    Request/Test Included in:
    Request test
    Comments:
    Samples should be separated and the serum/plasma stored in the freezer once it reaches Vetnostics. Oestrone (estrone) sulphate is a steroid hormone detectable in the circulation in many species. It can be produced in the gonads, the adrenals and is produced by the foetal-placental unit during pregnancy. Pregnancy >80 days: Oestrone sulphate levels rise during pregnancy and are a good indicator of a viable pregnancy. In mares oestrone sulphate is detectable in the maternal circulation from early as 60 days post-mating until foaling. In the event of foetal death oestrone sulphate disappears rapidly from circulation. Cryptorchidism in horses 3 yo or older. Goat pregnancy >50 days post-service
    Date Updated:
    1/10/2007
    Premating Swab (bitch, dog)
    Suitable Sample:
    Swab (vaginal)
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    24 hours for culture
    48 hours for sensitivity with routine organisms
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Primidone
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    Date Updated:
    1/10/2007
    Production Animal Profile
    Suitable Sample:
    EDTA
    AND
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL EDTA
    AND
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    FBC, Fibrinogen, Na, K, Cl, HCO3, Ca, Mg, PO4, AST, CPK, ALP, GGT, Urea, creatinine, glucose, T.Bil, T.Protein, Alb, Glob, (BHOB, C.Bil if necessary).
    Date Updated:
    30/06/2008
    Progesterone
    Suitable Sample:
    SERUM
    Values in gel serum tubes may increase if stored in the tube
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    2.5 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    Oestrus staging in bitches
    Early pregnancy in mares
    Pregnancy in camelids (collect samples 15-20 days after removal of male)
    Pregnancy in sows
    Date Updated:
    1/10/2007
    Protein (total protein)
    Suitable Sample:
    SERUM - serum protein
    LH - plasma protein
    EDTA - plasma protein
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    Date Updated:
    1/10/2007
    Protein electrophoresis
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    One (1) day - Monday to Friday
    Request/Test Included in:
    Request Test
    Comments:
    Used primarily to differentiate polyclonal from monoclonal gammopathies.
    Date Updated:
    1/10/2007
    Prothrombin Time (PT)
    Suitable Sample:
    CIT
    Unsuitable Sample:
    SERUM
    EDTA
    LH
    Minimum Sample Size:
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Beware of insufficiently filled OR overfilled CIT tubes; it is important to fill exactly to the mark on the tubes.
    Beware of expired tubes.
    Date Updated:
    1/10/2007
    Psittacine Beak and Feather Disease (PBFD, Circovirus) by PCR
    Suitable Sample:
    At least 3 freshly plucked feathers. These should be sealed in a small plastic bag or container.
    and/or
    LH blood: the volume should reflect the size of the bird, only a few drops are required for smaller species.
    Unsuitable Sample:
    EDTA
    SERUM
    Minimum Sample Size:
    Blood: volume of LH blood depends on size of bird, a few drops from smaller species is acceptable
    Feathers: at least 3 freshly plucked feathers (send in small plastic bag or container).
    A few drops of LH blood is acceptable from small species
    Turnaround Time:
    Approximately 2 weeks (confirm with laboratory)
    Request/Test Included in:
    Request test
    Comments:
    PCR test
    Date Updated:
    1/10/2007
    PT and APTT
    Suitable Sample:
    CIT
    Unsuitable Sample:
    SERUM
    EDTA
    LH
    Minimum Sample Size:
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Beware of insufficiently filled OR overfilled CIT tubes; it is important to fill exactly to the mark on the tubes.
    Beware of expired tubes.
    Date Updated:
    1/10/2007
    PTH (Parathyroid Hormone)
    Suitable Sample:
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Request/Test Included in:
    Comments:
    From October 2016 this test will no longer be available. We are evaluating the validity of alternative test methods and will notify clients when a suitable alternative is available.
    Date Updated:
    1/11/2016
    PTH-related Protein (Parathyroid Hormone-related Protein, PTHrp)
    Suitable Sample:
    EDTA tube with 200 uL Trasylol (see comments)
    Unsuitable Sample:
    SERUM, LH, SC
    Minimum Sample Size:
    At least 2.0 mL whole blood
    Turnaround Time:
    Seven (7) working days.
    Request/Test Included in:
    Request Test, contact laboratory in advance.
    Comments:
    This is NOT the same as PTH
    To collect the sample, hospitalise, fast overnight and collect 2 - 5 ml blood into a chilled plastic EDTA tube with 200 uL Trasylol.
    The sample must be centrifuged immediately and the plasma transferred to a chilled plain tube.
    If sufficient volume, split the sample and keep a frozen duplicate plasma at the clinic.
    Freeze ASAP, plasma must not thaw! Freeze overnight before sending in.
    Date Updated:
    1/10/2007
    PTT (partial thromboplastin time, APTT)
    Suitable Sample:
    CIT
    Unsuitable Sample:
    SERUM
    EDTA
    LH
    Minimum Sample Size:
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Beware of insufficiently filled OR overfilled CIT tubes; it is important to fill exactly to the mark on the tubes.
    Beware of expired tubes.
    Date Updated:
    1/10/2007
    Quantitative Electrophoresis (serum protein electrophoresis)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    Minimum Sample Size:
    2mL whole blood
    Turnaround Time:
    One (1) day - Monday to Friday
    Request/Test Included in:
    Request Test
    Comments:
    Used primarily to differentiate polyclonal from monoclonal gammopathies.
    Date Updated:
    1/10/2007
    Rabies Vaccination titre (for export)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    CIT
    FL
    Minimum Sample Size:
    >1 ml whole blood
    Turnaround Time:
    Fifteen (15) Working days
    Request/Test Included in:
    Request Test
    Comments:
    This is for animals travelling under the Petscheme which covers those travelling to the UK
    A correct AQIS form is required to be submitted.
    Date Updated:
    1/10/2007
    Relaxin (pregnancy in the bitch)+
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    CIT
    LH
    FL
    Minimum Sample Size:
    2mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Sample should be collected at 30 days +
    This test can be used in the cat
    Date Updated:
    8/07/2008
    Respiratory Panel - Canine
    Suitable Sample:
    Respiratory wash in EDTA, dry or gel swabs
    Unsuitable Sample:
    Wooden shaft swabs
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Canine Respiratory PCR Panel: - Bordetella bronchiseptica - Streptococcus equi subsp. zooepidemicus - Canine Adenovirus type 2 - Canine Herpesvirus type 1 - Canine Coronavirus - Canine Parainfluenza virus - Mycoplasma cynos PCR is a highly sensitive and specific method of testing for infectious canine Upper Respiratory Tract Disease (URTD). Sampling should ideally be performed early in the disease course. Increased chronicity, previous treatment and prior vaccination can reduce the expression of organisms on epithelial cells. The best samples for Canine Respiratory PCR contain large numbers of epithelial cells with minimal other material. Swabs with large amounts of ocular or nasal discharge may not be diagnostic. Sampling sites should be selected based on the clinical presentation, and may include nasal swabs, deep pharyngeal swabs, ocular swabs and swabs or fluid from a trans-tracheal wash (TTW) or bronchoalveolar lavage (BAL). The swabs should be placed in a single sterile container. The swab stems can be shortened using sterile scissors to fit the swabs into the tube. Do not place swabs in bacterial culture transport media. Label the container and keep the samples cool prior to sending to the laboratory for testing. If culture is also required, please submit a separate swab in transport media. If cytology is also required for TTW or BAL samples, please submit separate EDTA fluid and slides.
    Date Updated:
    30/06/2016
    Respiratory Panel - Equine
    Suitable Sample:
    Respiratory wash in EDTA, dry or gel swabs
    Unsuitable Sample:
    Wooden shaft swabs
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Equine Respiratory PCR Panel: - Equine Herpesvirus 1 - Equine Herpesvirus 4 - Rhodococcus equi - Streptococcus equi subspecies equi - Chlamydia psittaci
    Date Updated:
    30/06/2016
    Respiratory Panel - Feline
    Suitable Sample:
    Gel or dry swab/s (oropharyngeal and conjunctival) in sterile container
    Unsuitable Sample:
    Wooden shaft swabs
    Minimum Sample Size:
    Turnaround Time:
    2D
    Request/Test Included in:
    Comments:
    Feline Respiratory PCR Panel:
    - Feline Calicivirus
    - Bordetella bronchiseptica
    - Chlamydia felis
    - Feline Herpesvirus 1
    - Mycoplasma felis
    - Cryptococcus spp.


    PCR is the gold standard of testing for infectious feline Upper Respiratory Tract Disease (URTD).

    Population studies have been performed in several countries with similar results, with PCR prevalence of organisms in cats with infectious URTD approximately:
    Mycoplasma felis 45%
    Feline calicivirus 45%
    Feline herpesvirus 25%
    Chlamydia felis 15%
    Mixed infections 40%.


    Sampling should ideally be performed early in the disease course. Increased chronicity, previous treatment and prior vaccination can reduce the detection of organisms by reducing their expression on epithelial cells.

    Latent infection of Herpesvirus cannot be detected by PCR as the virus is sequestered in nerve ganglions and is not expressed on the epithelium.

    The best samples for Feline Respiratory PCR contain large numbers of epithelial cells with minimal other material. Swabs with large amounts of ocular or nasal discharge may not be diagnostic.

    Use sterile dry swabs to collect samples. The presence of ointment or other medications in the eye can prevent adequate epithelial collection and interfere with the PCR test and should be avoided. Thoroughly swab the conjunctiva, collecting from both eyes if possible. If nasal discharge is present, roll a sterile dry swab firmly along the nasal planum. A final swab should be used to collect a deep pharyngeal sample.

    If only a single site can be collected, a deep pharyngeal swab is preferred, however collecting from all three sites increases detection of organisms.

    The swabs should be placed in a single sterile container. The swab stems can be shortened using sterile scissors to fit the swabs into the tube. Do not place swabs in bacterial culture transport med
    Date Updated:
    30/06/2016
    Rheumatoid Factor - Canine
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    Positive in approximately 20-70% of rheumatoid arthritis cases
    Can get weak nonspecific positives in other illnesses
    Date Updated:
    1/10/2007
    Rodenticide (anticoagulant rodenticides Brodificoum and Flocoumafin)
    Suitable Sample:
    For postmortem samples liver is the best sample (preferably frozen) Vomitus - preferably frozen
    Unsuitable Sample:
    Blood Urine
    Minimum Sample Size:
    10 gm minimum, but the more the better
    Turnaround Time:
    Allow >6 weeks (contact laboratory to confirm expected time for availability of results).
    Request/Test Included in:
    Request test
    Comments:
    Quantitative assays for brodifacoum, bromodiolone, warfarin, coumatetrallyl and pindone
    Date Updated:
    1/10/2007
    Ross River Virus Antibody Titre
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    CIT
    FL
    Minimum Sample Size:
    >4 mL whole blood
    Turnaround Time:
    Five (5) working days
    Request/Test Included in:
    Request test
    Comments:
    IgG, IgM titres given
    Date Updated:
    21/07/2008
    Rotavirus Antigen in Faeces
    Suitable Sample:
    Fresh faeces
    If necessary, these can be stored for <14 days at 2-8°C
    Unsuitable Sample:
    Swab
    Blood
    Urine
    Minimum Sample Size:
    1-2 gm of faeces (small volume)
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request Test
    Comments:
    This test can be used on all animal species.
    Rotavirus is a common cause of severe diarrhoea in young domestic animals, birds and humans.
    Date Updated:
    1/10/2007
    Salmonella culture
    Suitable Sample:
    Swab Faeces
    Unsuitable Sample:
    Dry swabs
    Minimum Sample Size:
    Turnaround Time:
    Two (2) days
    Request/Test Included in:
    Request test
    Comments:
    Samples should be stored in a cool, dry place; samples do not need to be refrigerated.
    Samples stored at room temperature can be cultured for up to 3 days after collection
    Samples stored at 4°C can cultured for up to 4 days after collection.
    A fresh sample is more likely to yield a positive result
    Date Updated:
    1/10/2007
    Salmonella ID
    Suitable Sample:
    Swab
    Culture plate
    MacConkey agar plate
    Unsuitable Sample:
    Dry swabs
    Minimum Sample Size:
    Turnaround Time:
    Ten (10) Working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Selenium
    Suitable Sample:
    LH
    SERUM
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    10 mL whole blood
    5 - 10 mL milk
    100 gm fresh tissue (preferably liver)
    Feed - 10 gm minimum, 200 gm preferred
    Turnaround Time:
    Run Tuesdays and Thursdays
    Request/Test Included in:
    Request test
    Comments:
    Selenium in blood is useful in alpacas
    Whole blood is preferable to plasma as plasma only indicates current Se intake
    If plasma Se is required, it is better to centrifuge and separate off the plasma into a plain tube before sending the sample to the laboratory
    Date Updated:
    1/10/2007
    Serum iron
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Serum protein electrophoresis+
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    Minimum Sample Size:
    2mL whole blood
    Turnaround Time:
    One (1) day - Monday to Friday
    Request/Test Included in:
    Request Test
    Comments:
    Used primarily to differentiate polyclonal from monoclonal gammopathies.
    Date Updated:
    1/10/2007
    Snake Venom Detection
    Suitable Sample:
    Bite site swab - optimal sample
    Urine - preferred sample if bite site swab not available (see comments)
    Blood (EDTA) but is more likely to give false positive results
    Unsuitable Sample:
    SERUM
    Minimum Sample Size:
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    CATS collect urine 4-30 hours post-envenomation for best results
    DOGS collect urine <40-48 hours post-envenomation for best results
    Date Updated:
    1/10/2007
    Sodium (Na)
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    FL
    CIT
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Specific Gravity
    Suitable Sample:
    Urine
    Unsuitable Sample:
    Minimum Sample Size:
    0.5 mL minimum (SG alone)
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Strychnine
    Suitable Sample:
    Stomach contents
    Urine
    Unsuitable Sample:
    Blood
    Minimum Sample Size:
    5-10 mL if submitting urine
    Turnaround Time:
    Three (3) Working days
    Request/Test Included in:
    Request test
    Comments:
    This is detected as part of our general toxicological screening test for stimulants and depressants.
    Date Updated:
    25/07/2008
    Synovial Fluid Cytology + Analysis
    Suitable Sample:
    Direct smear; this is important sample for cytology
    SERUM/sterile pot if sample is to be cultured (EDTA is not suitable for culture)
    EDTA is better for cytology alone (see comment)
    Unsuitable Sample:
    Minimum Sample Size:
    0.5 mL for cell count is to be done
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    If sufficient fluid, place some in an EDTA tube and the rest in either a SERUM tube (without activator) or a sterile urine container.
    Date Updated:
    1/10/2007
    T4
    Suitable Sample:
    SERUM (canine, feline, equine)
    Unsuitable Sample:
    EDTA
    FL
    LH
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Adding a canine TSH for canine samples increases the sensitivity but not the specificity.
    Date Updated:
    1/10/2007
    T4 - free by equilibrium dialysis (canine)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Five (5) working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    T4 + Cholesterol
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    FL
    LH
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Adding a canine TSH for canine samples increases the sensitivity but not the specificity.
    Date Updated:
    1/10/2007
    Taurine
    Suitable Sample:
    SERUM
    Lith Hep PLASMA
    Unsuitable Sample:
    Minimum Sample Size:
    1ml serum, plasma
    Turnaround Time:
    Seven (7) working days
    Request/Test Included in:
    Request test
    Comments:
    Serum/Plasma must be separated and frozen immediately
    Transport to laboratory frozen
    Only published, or expected, reference ranges are available
    Date Updated:
    21/07/2008
    Testosterone
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    2.5 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    The hCG response test is used to diagnose rigs in horses <3 yo (use oestrone sulphate in horses 3 yo or older).
    The hCG response test is also used in mules and other animals.
    Date Updated:
    1/10/2007
    Thiamine (RBC)
    Suitable Sample:
    EDTA
    Unsuitable Sample:
    LH
    Minimum Sample Size:
    >0.5 mL whole blood
    Turnaround Time:
    Turnaround time 7d
    Request/Test Included in:
    Request test
    Comments:
    Freeze EDTA blood and protect from light by wrapping tube with foil ASAP.
    Must not be on antibiotics
    Red cell thiamine is a measure of stores and best for animal assessment.
    Serum thiamine will reflect feeding over the last few days.
    Date Updated:
    21/07/2008
    TLI (Canine)
    Suitable Sample:
    SERUM (canine only) An overnight (>6 hours) fast is recommended prior to blood collection
    Unsuitable Sample:
    EDTA LH FL CIT Species other than canine
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Run Monday, Wednesday and Friday Results available the same day
    Request/Test Included in:
    Request test
    Comments:
    Sensitive and specific test for the diagnosis of canine exocrine pancreatic insufficiency (EPI) Reference range for healthy dogs is 5.4 - 32 ng/mL Values <2.5 ng/mL are diagnostic for canine exocrine pancreatic insufficiency. Dogs with values 2.5 - 5.0 ng/mL should be retested in one month. Administration of oral pancreatic extracts does not affect serum TLI concentrations in normal dogs or dogs affected with EPI. Consequently withdrawal of enzyme supplementation is unnecessary.
    Date Updated:
    18/07/2008
    Total Protein (serum/plasma protein)
    Suitable Sample:
    SERUM - serum protein
    LH - plasma protein
    EDTA - plasma protein
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Total T4 (canine, feline, equine)
    Suitable Sample:
    SERUM (canine, feline, equine)
    Unsuitable Sample:
    EDTA
    FL
    LH
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Adding a canine TSH for canine samples increases the sensitivity but not the specificity.
    Date Updated:
    1/10/2007
    Toxoplasma Antibody Titre IgM
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    Samples from species other than dogs and cats
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Five (5) working days
    Request/Test Included in:
    Request Toxo IgM
    Comments:
    The IgM titre is used to suggest current/active disease
    Date Updated:
    1/10/2007
    Toxoplasma Antibody titres by IFA (dogs and cats - IgG and IgM)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    Samples from species other than dogs and cats
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Five (5) working days
    Request/Test Included in:
    RequestToxo IgG and IgM
    Comments:
    The IgG titre is used to suggest previous exposure
    The IgM titre is used to suggest current/active disease
    Date Updated:
    1/10/2007
    Toxoplasma Latex Agglutination Screening Test (all species)
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    LH
    EDTA
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Run Monday, Wednesday and Friday
    Results available the same day
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Trace Elements x 3
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Three (3) working days
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Tracheal Wash Cytology
    Suitable Sample:
    Tracheal wash sample in sterile container (no anticoagulant)
    Unsuitable Sample:
    Minimum Sample Size:
    0.5 mL
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Triglycerides
    Suitable Sample:
    SERUM
    LH (plasma may give lower values)
    EDTA
    Unsuitable Sample:
    FL
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Troponin I
    Suitable Sample:
    SERUM (gel tubes OK)
    LH (will give slightly lower results than serum)
    If monitoring, it is important to use the same sample type and same type of tubes for each sample
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    1.0 mL whole blood
    Turnaround Time:
    Daily - Monday - Friday
    Request/Test Included in:
    Request test
    Comments:
    This is presently an unvalidated test in equine, canine and feline.
    Troponin I in serum increases in response to myocardial injury.
    Date Updated:
    26/07/2008
    Trypsin-like Immunoreactivity (TLI) - Canine
    Suitable Sample:
    SERUM (canine only)
    An overnight (>6 hours) fast is recommended prior to blood collection
    Unsuitable Sample:
    EDTA
    LH
    FL
    CIT
    Species other than canine
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    Run Monday, Wednesday and Friday
    Results available the same day
    Request/Test Included in:
    Request test
    Comments:
    Sensitive and specific test for the diagnosis of canine exocrine pancreatic insufficiency (EPI)
    Reference range for healthy dogs is 5.4 - 32 ng/mL
    Values <2.5 ng/mL are diagnostic for canine exocrine pancreatic insufficiency.
    Dogs with values 2.5 - 5.0 ng/mL should be retested in one month.
    Administration of oral pancreatic extracts does not affect serum TLI concentrations in normal dogs or dogs affected with EPI. Consequently withdrawal of enzyme supplementation is unnecessary.
    Date Updated:
    18/07/2008
    TSH - canine thyroid stimulating hormone
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    2.0 mL whole blood
    Turnaround Time:
    Monday to Friday
    Request/Test Included in:
    Request test
    Comments:
    In approximately 70% of true hypothyroid dogs there is elevated TSH.
    Date Updated:
    1/10/2007
    Tying Up - FEE Prof.
    Suitable Sample:
    SERUM and URINE both needed
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    Two (2) days
    Request/Test Included in:
    Request test
    Comments:
    Used in horses to determine fractional electrolyte excretions of Na, K, Cl , PO4 and Mg.
    Ca not determined due to difficulty in obtaining representative urine sample.
    Date Updated:
    26/07/2008
    Urea
    Suitable Sample:
    SERUM (preferred)
    LH
    EDTA
    Unsuitable Sample:
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Uric Acid
    Suitable Sample:
    SERUM (preferred)
    LH
    EDTA
    Unsuitable Sample:
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Urinalysis (biochemistry + SG + wet microscopy)
    Suitable Sample:
    Urine
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL minimum, but >5 mL is much better
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Urinalysis = biochemistry + SG + wet microscopy
    Date Updated:
    1/10/2007
    Urine Cortisol:Creatinine Ratio
    Suitable Sample:
    Urine
    Unsuitable Sample:
    Blood
    Minimum Sample Size:
    2 mL minimum, but >5 mL is much better
    Turnaround Time:
    Monday and Thursday
    Request/Test Included in:
    Request test
    Comments:
    Screening test to help rule out hyperadrenocorticism.
    Date Updated:
    1/10/2007
    Urine Culture & Sensitivity (quantitative, aerobic)
    Suitable Sample:
    Urine by cystocentesis is optimal
    Urine via catheter
    Voided sample
    Please state how sample collected
    Unsuitable Sample:
    Urine with obvious faecal contamination (eg from dirty litter tray)
    Minimum Sample Size:
    2 mL minimum, but >5 mL is much better
    Turnaround Time:
    Culture: Most organisms grown within 12 hours.
    Request/Test Included in:
    Request test
    Comments:
    If Aspergillus suspected please enter on request form
    Samples stored at 4°C cultured for up to 24 hours after collection give good results
    Samples stored at room temperature for up to 2 days and samples stored at 4°C for up to 3 days can be cultured, but both give less reliable results
    Room temperature results in overgrowth, 4°C may kill fastidious organisms
    Date Updated:
    26/07/2008
    Urine Culture & Sensitivity (quantitative, aerobic) + Urinanalysis
    Suitable Sample:
    Urine by cystocentesis is optimal
    Urine via catheter
    Voided sample
    Please state how sample collected
    Unsuitable Sample:
    Urine with obvious faecal contamination (eg from dirty litter tray)
    Minimum Sample Size:
    2 mL minimum, but >5 mL is much better
    Turnaround Time:
    Culture: Most organisms grown within 12 hours.
    Request/Test Included in:
    Request test
    Comments:
    If Aspergillus suspected please enter on request form.
    Date Updated:
    26/07/2008
    Urine Cytology
    Suitable Sample:
    Urine
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL minimum, but >5 mL is much better
    Turnaround Time:
    24 hours Mon-Sat
    Request/Test Included in:
    Request test
    Comments:
    Urine cytology is indicated specifically if neoplasia is suspected, but is not usually necessary for the diagnosis of urinary tract inflammation and/or infection.
    Date Updated:
    1/10/2007
    Urine Protein:Creatinine Ratio
    Suitable Sample:
    Urine
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL minimum, but >5 mL is much better
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Quantitates urinary protein loss
    Date Updated:
    1/10/2007
    Urine SG (specific gravity)
    Suitable Sample:
    Urine
    Unsuitable Sample:
    Minimum Sample Size:
    0.5 mL minimum (SG alone)
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Vaccination Status - Canine Distemper Virus & Parvovirus Antibody Titres by IFA
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    EDTA
    LH
    Minimum Sample Size:
    1 mL whole blood
    Turnaround Time:
    8 working days
    Request/Test Included in:
    Combined CDV & CPV IFA titres
    The tests can also be requested individually
    Comments:
    Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations. Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.
    Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests. Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV.
    INTERPRETATION:
    Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
    Titres of >1:5 are considered seropositive
    Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
    Dogs with titres of >1:20 probably do not require revaccination.
    Date Updated:
    27/08/2008
    Vaginal cytology
    Suitable Sample:
    Air-dried smear(s)
    OR
    Swab
    Unsuitable Sample:
    Minimum Sample Size:
    Single smear
    Turnaround Time:
    24 hours Monday-Saturday
    Request/Test Included in:
    Request test
    Comments:
    May be used in conjunction with serum progesterone.
    Date Updated:
    1/10/2007
    Vaginal swab (premating)
    Suitable Sample:
    Swab (vaginal)
    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    24 hours for culture
    48 hours for sensitivity with routine organisms
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    VBA
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA (can do some tests, see comments)
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    If only EDTA is submitted:
    Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
    Tests that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorus, HCO3
    Date Updated:
    1/10/2007
    VBA + Amylase
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA (can do some tests, see comments)
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    If only EDTA is submitted:
    Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
    Tests that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorus, HCO3
    Date Updated:
    1/10/2007
    VBA + Amylase + Lipase
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA (can do some tests, see comments)
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    If only EDTA is submitted:
    Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
    Tests that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorus, HCO3
    Date Updated:
    1/10/2007
    VBA + FBC
    Suitable Sample:
    EDTA
    SERUM
    Unsuitable Sample:
    EDTA (cano do some tests, see comments)
    Minimum Sample Size:
    2 mL whole blood
    EDTA
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    If only EDTA is submitted:
    Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
    Tests that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorus, HCO3
    Date Updated:
    1/10/2007
    VBA + FBC + Amylase
    Suitable Sample:
    EDTA
    SERUM
    Unsuitable Sample:
    EDTA (can do some tests, see comments)
    Minimum Sample Size:
    2 mL whole blood
    EDTA
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    If only EDTA is submitted:
    Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
    Test that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorus, HCO3
    Date Updated:
    1/10/2007
    VBA + FBC + Amylase + Lipase
    Suitable Sample:
    EDTA
    SERUM
    Unsuitable Sample:
    EDTA (can do some tests, see comments)
    Minimum Sample Size:
    2 mL whole blood
    EDTA
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    If only EDTA is submitted:
    Tests that CAN be done: AST, albumin, ALT, bile acids, bilirubin, BOHB, cholesterol, creatinine, GGT, glucose, lipase, total protein, triglycerides, urea, uric acid
    Tests that CANNOT be done: ALP, amylase, calcium, CK, chloride, GLDH, potassium, magnesium, sodium, phosphorus, HCO3
    Date Updated:
    1/10/2007
    Vitamin A
    Suitable Sample:
    LH
    Unsuitable Sample:
    SERUM
    EDTA
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Ten (10) working days, but verify with the laboratory
    Request/Test Included in:
    Request test
    Comments:
    Plasma must be separated immediately, wrapped in foil and kept frozen
    Transport to laboratory frozen
    Only published, or expected, reference ranges are available
    Date Updated:
    1/10/2007
    Vitamin A and E
    Suitable Sample:
    LH
    Unsuitable Sample:
    SERUM
    EDTA
    FL
    Minimum Sample Size:
    4 mL whole blood
    Turnaround Time:
    Ten (10) working days, but verify with the laboratory
    Request/Test Included in:
    Request tests
    Comments:
    Plasma must be separated immediately, wrapped in foil and kept frozen
    Transport to laboratory frozen
    Only published, or expected, reference ranges are available
    Date Updated:
    1/10/2007
    Vitamin B12 for Large Animals (Cobalt)
    Suitable Sample:
    LH for MMA in plasma
    Milk
    Tissue
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    Minimum of 2 mL of whole blood for plasma MMA
    5-10 mL of milk
    100 gm of fresh tissue, preferably liver
    Turnaround Time:
    2 weeks
    Request/Test Included in:
    Request test
    Comments:
    Vitamin B12 is used to measure cobalt status as a deficiency of cobalt results in a deficiency of vitamin B12. In ruminants vitamin B12 is measured as MMA (methylmalonic acid); measurement of plasma MMA is the preferred assay for vitamin B12 status in cattle (vitamin B12 is necessary for the normal metabolism of MMA). Vitamin B12 deficiency results in elevated MMA levels.
    Date Updated:
    1/10/2007
    Vitamin B12 for Small Animals
    Suitable Sample:
    SERUM
    LH
    Unsuitable Sample:
    EDTA
    Minimum Sample Size:
    1.5 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Vitamin E
    Suitable Sample:
    LH
    Unsuitable Sample:
    SERUM
    EDTA
    FL
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Ten (10) working days, but verify with the laboratory
    Request/Test Included in:
    Request test
    Comments:
    Plasma must be separated immediately, wrapped in foil and kept frozen
    Transport to laboratory frozen
    Only published, or expected, reference ranges are available
    Date Updated:
    1/10/2007
    von Willebrand Factor
    Suitable Sample:
    CIT (1 part citrate to 9 parts blood) Fast 12 hours prior to collection, must be a resting sample. See Comments below for more detailed information.
    Unsuitable Sample:
    EDTA SERUM LH
    Minimum Sample Size:
    4 mL whole blood filled precisely to line or mark on citrate tube.
    Turnaround Time:
    Eight (8) working days.
    Request/Test Included in:
    Request test
    Comments:
    Beware of insufficiently filled OR overfilled CIT tubes and expired tubes. Separate and freeze the plasma ASAP(preferred), but citrated whole blood can be kept chilled and then centrifuged with plasma separated and frozen in <24 hours. If separating off plasma, do NOT transfer to tubes which have clot activator (gel or powder) in them. Frozen plasma is stable for <1 month and must remain frozen during transit REQUEST DRY ICE courier pickup. Recommend prepare duplicate frozen plasma samples so one can be kept in reserve at vet practice. Please attach original citrate tube to the tube containing separated plasma on submission.
    Date Updated:
    29/09/2008
    WBC & Differential Count (White blood cell count & differential)
    Suitable Sample:
    EDTA
    CIT/FL if less than 1 day old
    LH for some birds and reptiles
    Unsuitable Sample:
    SERUM
    Minimum Sample Size:
    1.0 mL, but prefer 2 mL
    If smear only, WCC will be estimated from the smear
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    LH can be used for this test , but platelets and WBCs clump.
    Date Updated:
    1/10/2007
    Wellness Profile
    Suitable Sample:
    SERUM
    Unsuitable Sample:
    Minimum Sample Size:
    2 mL whole blood
    Turnaround Time:
    Daily
    Request/Test Included in:
    Request test
    Comments:
    Date Updated:
    1/10/2007
    Zinc (large animals)
    Suitable Sample:
    Special Trace element tube (contact lab)
    Tissue - liver if deficiency suspected
    Tissue - liver or kidney if excess is suspected
    Unsuitable Sample:
    Hemolyzed samples interfere with assay.
    Minimum Sample Size:
    1 mL whole blood
    100 gm fresh tissue (can be frozen)
    Turnaround Time:
    Approx 3 days for whole blood and 10 working days for tissue
    Request/Test Included in:
    Request test
    Comments:
    Samples in vacutainers are not suitable as the stoppers contain zinc.
    Date Updated:
    6/08/2008
    Zinc (small animals, birds)
    Suitable Sample:
    Special Trace element tube(contact lab)
    Unsuitable Sample:
    Hemolyzed samples interfere with assay
    Minimum Sample Size:
    1 ml whole blood
    Turnaround Time:
    Approximately 3 days
    Request/Test Included in:
    Request test
    Comments:
    Samples in vacutainers are not suitable as the stoppers contain zinc.
    Date Updated:
    6/08/2008